Paul Willis to Provide Experimental Methodology for AZ THP1 cytotox assay

mattodd commented 10 years ago

AstraZeneca recently provided hERG data for some members of Series 4. http://malaria.ourexperiment.org/biological_data/11081 The assay uses methodology described in this paper: 10.1016/j.vascn.2006.02.003 The THP1 cyctotox assay requires a protocol so we can write up the work. @PaulWillisMMV to chase this, after which it will be posted to the lab notebook.

alintheopen commented 10 years ago

Hi @mattodd and @PaulWillisMMV we could really do with this protocol asap as @JoannaUbels and @tscmacdonald need to include details in their theses (due very soon!) Thanks, Alice

PaulWillisMMV commented 10 years ago

I will ask AZ again for their protocol. In the event that It does not arrive in time I would suggest just leaving this cytotoxicity data out of the thesis. I would not see it as essential information for a chemistry based thesis

Paul

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On 23 Oct 2014, at 05:30, "alintheopen" notifications@github.com<mailto:notifications@github.com> wrote:

Hi @mattoddhttps://github.com/mattodd and @PaulWillisMMVhttps://github.com/PaulWillisMMV we could really do with this protocol asap as @JoannaUbelshttps://github.com/JoannaUbels and @tscmacdonaldhttps://github.com/tscmacdonald need to include details in their theses (due very soon!) Thanks, Alice

Reply to this email directly or view it on GitHubhttps://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/264#issuecomment-60188419.

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mattodd commented 10 years ago

Thanks for chasing, guys. If it's strongly related to a published method, we can just cite a paper, too.

On 23 October 2014 07:09, PaulWillisMMV notifications@github.com wrote:

I will ask AZ again for their protocol. In the event that It does not arrive in time I would suggest just leaving this cytotoxicity data out of the thesis. I would not see it as essential information for a chemistry based thesis

Paul

Sent from my iPhone

On 23 Oct 2014, at 05:30, "alintheopen" <notifications@github.com<mailto: notifications@github.com>> wrote:

Hi @mattoddhttps://github.com/mattodd and @PaulWillisMMV< https://github.com/PaulWillisMMV> we could really do with this protocol asap as @JoannaUbelshttps://github.com/JoannaUbels and @tscmacdonald< https://github.com/tscmacdonald> need to include details in their theses (due very soon!) Thanks, Alice

Reply to this email directly or view it on GitHub< https://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/264#issuecomment-60188419>.

This email and any attachments may contain confidential information. If you are not the intended recipient, please delete the email and any attachments and notify the sender immediately.

— Reply to this email directly or view it on GitHub https://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/264#issuecomment-60196492 .

MATTHEW TODD | Associate Professor School of Chemistry | Faculty of Science

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PaulWillisMMV commented 10 years ago

The scientist is on holiday so the protocol will not be available any time soon. I do not know which method they used to determine cell viability so cannot reference a paper. I strongly suggest that this assay is not key in the great work carried out for the thesis and that there will be no impact on the overall conclusions if the assay result is omitted

Paul

Paul Willis| Medicines for Malaria Venture Director, Drug Discovery Tel: +41 22 555 03 42 Mobile: +41 79 102 78 74 Email: Willisp@mmv.orgmailto:Willisp@mmv.org

Defeating Malaria Together | www.mmv.orghttp://www.mmv.org/

From: Mat Todd [mailto:notifications@github.com] Sent: Thursday, 23 October 2014 11:41 AM To: OpenSourceMalaria/OSM_To_Do_List Cc: Paul Willis Subject: Re: [OSM_To_Do_List] Paul Willis to Provide Experimental Methodology for AZ THP1 cytotox assay (#264)

Thanks for chasing, guys. If it's strongly related to a published method, we can just cite a paper, too.

On 23 October 2014 07:09, PaulWillisMMV notifications@github.com wrote:

I will ask AZ again for their protocol. In the event that It does not arrive in time I would suggest just leaving this cytotoxicity data out of the thesis. I would not see it as essential information for a chemistry based thesis

Paul

Sent from my iPhone

On 23 Oct 2014, at 05:30, "alintheopen" <notifications@github.com<mailto: notifications@github.com>> wrote:

Hi @mattoddhttps://github.com/mattodd and @PaulWillisMMV< https://github.com/PaulWillisMMV> we could really do with this protocol asap as @JoannaUbelshttps://github.com/JoannaUbels and @tscmacdonald< https://github.com/tscmacdonald> need to include details in their theses (due very soon!) Thanks, Alice

Reply to this email directly or view it on GitHub< https://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/264#issuecomment-60188419>.

This email and any attachments may contain confidential information. If you are not the intended recipient, please delete the email and any attachments and notify the sender immediately.

— Reply to this email directly or view it on GitHub https://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/264#issuecomment-60196492 .

MATTHEW TODD | Associate Professor School of Chemistry | Faculty of Science

CRICOS 00026A This email plus any attachments to it are confidential. Any unauthorised use is strictly prohibited. If you receive this email in error, please delete it and any attachments.

— Reply to this email directly or view it on GitHubhttps://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/264#issuecomment-60215186.

This email and any attachments may contain confidential information. If you are not the intended recipient, please delete the email and any attachments and notify the sender immediately.

alintheopen commented 8 years ago

Hi @mattodd @PaulWillisMMV do we have any news on the assay - just updating the wiki, and this would be good to have. Cheers Alice

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Paul Willis to Provide Experimental Methodology for AZ THP1 cytotox assay #264