Closed mattodd closed 6 years ago
@mattodd Interesting idea and I think worth trying beyond just as a proof of concept with regards to the maintenance of activity. I have convinced myself that the primary metabolic hotspot is the pyrazine ring of the triazolopyrazine core and not necessarily the tethered ethoxyphenyl side chain of the various analogs that have been synthesized. Testing your proposed analog (whether active or not) for metabolic stability might definitively inform us whether or not the liability is the core itself versus the side chain.
While on the topic of unusual functional groups in drugs - Could replacing the difluromethoxy group with a TMS be in order?
TMS ethers of phenols are pretty labile.
Chris
On 18 Apr 2016, at 15:51, MFernflower notifications@github.com wrote:
While on the topic of unusual functional groups in drugs - Could replacing the difluromethoxy group with a TMS be in order?
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Oops! I explained it all wrong! Below is an image (properly) explaining my idea:
https://cloud.githubusercontent.com/assets/3164942/14618362/483ff36c-0580-11e6-88db-e5683bbb1a83.png
But why, Mandrake? If you suggest an alternative, it helps everyone if you can provide a Why. Is there a reason why you think this might be advantageous, based on what's known in the literature? Is it likely such a replacement would solve a problem we currently face, i.e. improving potency, improving solubility and/or slowing metabolic clearance? The cubane replacement does seem to have such advantages, which is the reason I suggested it. What might a TMS switch solve that means this suggestion should be prioritised over others? If there are reasons (and there may well be) do you have in mind a lab with experience in making such compounds that might be interested in making your compound, or do you have a way to contact any local NY students with the bandwidth to do so?
On 19 April 2016 at 05:01, MFernflower notifications@github.com wrote:
Woops! I ment to say that replacing the entire OCHF2 group with TMS.
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You have quite valid points indeed! I will attempt to explain my current understanding and ideas around this class of molecules:
Chemdraw gives CLogP of the cubane analog as 4.7. I wonder if that's accurate. I had thought that deplanarisation would help, but perhaps I'm confusing lipophilicity with kinetic solubility. Grease is grease. I might see if Craig himself is interested in the synthesis of this molecule, or at least the cubane alcohol subunit.
Chemaxon gives logP 3.5 XlogP gives 3.8 OChem 2.3 alogP 1.9
The variability would suggest this sort of structure is outside the applicability domain of the algorithms
+1
On 28 April 2016 at 07:28, Chris Swain notifications@github.com wrote:
Chemaxon gives logP 3.5 XlogP gives 3.8 OChem 2.3 alogP 1.9
The variability would suggest this sort of structure is outside the applicability domain of the algorithms
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One of the features of the two aryl systems is that there is the possibility of hydrophobic collapse with the two aryl rings pi-stacking (this was seen in the NMR). It would be interesting to see how the proposed cubane behaves.
I should have checked - in the paper logP values are indirectly calculated from a combination of lit values (of benzene analogs) and HPLC retention times (of the benzene vs cubane analogs). This is described in the SI. The results suggest cubanes have similar logP values, but the effect in our case may be different, of course.
@mattodd
Shouldn't the cubane be replacing the benzene ring on the right-most side of the molecule? The phenyl on the ether linker has been manipulated quite a bit in various other compounds so doing this would provide a bit of contrast.
This is the best I came up with sofar: http://www.chemicalize.org/structure/#!mol=3-[4-%28difluoromethoxy%29cubyl]-5-%28imidazolylethoxy%29-[1%2C2%2C4]triazolo[4%2C3-a]pyrazine&source=calculate
It is also interesting to note that chemicalize's predictive algorithms seem to choke on any cubane containing drug @drc007
Certainly that's a possibility, yes. We'd need cubane aldehyde, which might be available. Though unsubstituted phenyl is no good in the northeast, so we'd need "para" substituted cubane aldehyde most likely. On 30 Apr 2016 9:57 am, "MFernflower" notifications@github.com wrote:
Shouldn't the cubane be replacing the benzene ring on the right-most side of the molecule? The aryl on the ether linker is quite easy to replace with something a little more common (furan, benzonitrile, imidazole, ETC) as it is thought that whatever is on the linker side does not really matter (hence the creation of a sulfoxide linker by Edwin)
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How would you bolt the difluoromethoxy onto the cubane?
Oh, I'm not sure it's worth the extra synthetic hassle of doing that at this stage. We know that 4-Cl and 4-CN etc work fine on phenyl, so we can go with one of those rather. It depends on what's synthetically accessible.
On 30 April 2016 at 10:15, MFernflower notifications@github.com wrote:
That does raise a good point - how would you bolt the difluoromethoxy onto the cubane?
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@mattodd I understand completely.. I also did not know that 4-chloro was an acceptable modification to the rightmost side! I'll keep that in mind when doing computations as it makes a few things slightly easier - I also just got a Linux laptop so this should allow me to do more detailed computations and whatnot. Also what did you think of the imidazolyl on my example molecule? Have 5 membered rings on the linker end been tested at all?
Hi Mandrake, you can browse the wiki or the spreadsheet for all the structure-activity data.
@mattodd This may have been discussed previously, so my apologies up front if that was the case. I was reviewing a few papers that I have been accumulating and focused in on a J.Med.Chem (2015) 58, pp. 8713-8722 paper on some antimalarial imidazopyridazines / pyrazolopyridines. Two of the more potent structures are shown below:
It started to make me think that the meta-sulfone may be contributing to activity in a similar manner to the analogs that have branched alcohols and amines (like MMV670947). It was also already demonstrated that the positional isomer on the triazolopyrazine ring system also can be active (MMV670945).
Since one of the biggest hurdles at the moment is the trend toward higher LogP's, I was wondering if we missed the opportunity to include the sulfone and sulfoxide analogs described in the paper. Would it make sense at this point to target a few analogs like the ones shown below? (I have only shown the sulfone-sulfoxide analogs, but the sulfone-sulfone could also be made. There was discussion on this in the previously mentioned paper.)
Edwin may be producing a few sulfonamides for testing (I chatted with him)
These initial analogs do not use the sulfonamide linker, they are sulfones or sulfoxides remote from the linker position.
O=S(C)C(C=C1)=CC=C1C(N23)=NN=C3C=NC=C2OCCC4=CC(S(C5CC5)(=O)=O)=CC=C4 O=S(C1=CC=CC(COC2=CN=CC3=NN=C(C4=CC=C(S(C)=O)C=C4)N23)=C1)(C5CC5)=O O=S(C)C(C=C1)=CC=C1C(N23)=NN=C3C=NC=C2OCCC4=C(S(C5CC5)(=O)=O)C=CC=C4 CS(C(C=C1)=CC=C1C(N23)=NN=C3C=NC=C2OCC4=C(S(C5CC5)(=O)=O)C=CC=C4)=O
@medchemprof @mattodd Thanks for the SMILES strings! Here are two ideas I came up with:
N1(CCCC1)S(=O)(=O)C1=C(C=CC=C1)CCOC1=CN=CC=2N1C(=NN2)C2=CC=C(C#N)C=C2 LogP: 1.87
C1(CC1)S(=O)(=O)C1=C(C=CC=C1)CN(S(=O)(=O)C1=CN=CC=2N1C(=NN2)C2=CC=C(C=C2)OC(F)F)C LogP: 1.85
Interesting idea @MedChemProf - synthetically that might be quite straightforward, and the impact in cLogP is dramatic. We should look at how easy it might be to make the required coupling fragments - the aldehyde for the northeast and the alcohol for the northwest. Could I ask that if we pursue this more that we separate this out to a separate Issue - we're supposed to be talking about cubane here...
Good news - Craig Williams has agreed to try to send us a sample of 2-cubylethanol which we can couple with the chloro precursor to make a final cubane analog. Craig says that the cubane aldehyde (for the northeast) is difficult to handle, but that the “para”-iodo carboxylic acid is available and we could reduce that to the aldehyde, which ought to be easier to handle. He’s offering to ship that acid too. These are valuable new inputs that should allow us to evaluate the effectiveness of cubanes in these compounds as phenyl isosteres. Craig is happy to share the experimental details of relevant transformations in the spirit of the open project.
Cubane samples have arrived in Sydney from Craig Williams. Synthesis should begin shortly - link will appear here when it does.
Synthesis using the cubane samples are in progress. See Issue #395.
With the interesting potency results received from the cubane bioisosteres (here) it was seen that we were lacking a comparison for the RHS replacement (OSM-S-375) and whether or not it was the size of the iodine that was the issue. The para iodo phenyl analogue has just been synthesised (ELN entry here) and will be sent for testing in the next batch.
Strings IC(C=C1)=CC=C1C2=NN=C3C=NC=C(OCCC4=CC(F)=C(F)C=C4)N32 InChI=1S/C19H13F2IN4O/c20-15-6-1-12(9-16(15)21)7-8-27-18-11-23-10-17-24-25-19(26(17)18)13-2-4-14(22)5-3-13/h1-6,9-11H,7-8H2 SYOXDOQMLHMQGB-UHFFFAOYSA-N
Could you just insert the string in the above, @edwintse , and the link to the latest ELN entry? Ta.
I was speaking with Craig Williams at UQ yesterday about his lovely cubane work, which largely validates cubane as a bioisostere of phenyl, and one with potentially lower metabolic liability. Surely we could incorporate this in the side chains of Series 4, given that we might be able to obtain derivatised cubane (e.g. the carboxylic acid) from CSIRO here in Aus.
One could imagine a number of ways of doing this, e.g. the most obvious
But there may be others. The replacement certainly helps with deplanarisation. What say you all?