Open edwintse opened 6 years ago
Fantastic work guys, this is a lovely set of compounds.
The results are in.
(For those that weren't online for the public meeting yesterday, these results came through during the meeting! Right on cue after I had concluded my section by saying we were awaiting the OHOH compound results that had arrived in Dundee, and just as Ed started summarizing our other recent potency results. Cue much excitement from the four of us huddled around our computers and scrambling to login and sift through the data. It wasn't quite as we hoped (see below), so for those that were present I hope it made sense as we tried to explain - it really was as live as possible on this occasion!)
Anyway, both runs of data are in (the second was uploaded an hour or so later) and the values below are the average of the two runs. Raw data is posted on my ELN, and data is linked on the wiki.
Some brief comments:
@david1597 Seeing the potency of the meta-obz got me thinking - has this molecule ever been screened?
I'd also be curious about it's herg effects since the carboxylic acid is there but in a different spot @edwintse
Thoughts everyone?
We have received extra repeats for that all important OHOH compound - OSM-S-556. The average potency is 660 nM with the four repeats being 425, 792, 817 and 601 nM. The curves for those four repeats are below. Ten data points are collected in each run but some data points appear to be missed off in some cases due to very low bottoms.
This value is not what we hoped or expected - it's a long way from the <10 nM that we got from the Pfizer biosynthesis. I'm unsure how to proceed with this. There's still a repeated biosynthesis sample we're awaiting the potency result for. And we will submit another sample of the DGS 81-1 batch to Dundee in the next shipment.
@David1597 @mcoster I wonder if the Pfizer 10 nm was a quinone? Could explain the efficacy - possibly killing cells via an oxidation mechanism along side atp4 blockade!
Here are all the individual repeat values for this batch:
MMV number | OSM number | Internal number | Potency 1 | Potency 2 | Potency 3 | Potency 4 | Average |
---|---|---|---|---|---|---|---|
MMV1580437 | OSM-S-552 | MK070-2 | >25.11 | >25.11 | >25 | ||
MMV1581295 | OSM-S-556 | DGS 81-1 | 0.425 | 0.792 | 0.817 | 0.601 | 0.659 |
MMV1581296 | OSM-S-558 | DGS 69-1 | >25.11 | >25.11 | >25 | ||
MMV1581297 | OSM-S-559 | DGS 74-1 | 0.165 | 0.133 | 0.149 | ||
MMV1581298 | OSM-S-560 | DGS 77-1 | 0.295 | 0.239 | 0.267 | ||
MMV1581299 | OSM-S-561 | DGS 78-1 | >25.11 | >25.11 | >25 | ||
MMV1581300 | OSM-S-562 | DGS 73-1 | 0.333 | 0.34 | 0.337 | ||
MMV1581301 | OSM-S-563 | DGS 84-1 | 18.32 | 20.88 | 19.600 | ||
MMV1581302 | OSM-S-565 | DGS 38-1 | >25.11 | >25.11 | >25 | ||
MMV1581303 | OSM-S-566 | DGS 13-2 | 3.93 | 3.768 | 3.849 | ||
MMV1581304 | OSM-S-567 | DGS 26-1 | 0.142 | 0.151 | 0.147 | ||
MMV1581305 | OSM-S-568 | DGS 82-1 | >25.11 | >25.11 | >25 | ||
MMV1581306 | OSM-S-569 | DGS 83-3 | >25.11 | >25.11 | >25 | ||
MMV1581335 | OSM-S-570 | EGT 48-2 (f5-7) | 6.57 | 4.674 | 5.622 | ||
MMV1581336 | OSM-S-571 | EGT 52-2 (f6-10) | 1.099 | 0.997 | 1.048 | ||
MMV1581337 | OSM-S-572 | EGT 347-1 | 14.76 | 14.15 | 14.455 | ||
MMV1581338 | OSM-S-573 | EGT 348-1 | >25.11 | >25.11 | >25 | ||
MMV1581339 | OSM-S-574 | EGT 349-1 | >25.11 | >25.11 | >25 | ||
MMV1581340 | OSM-S-575 | EGT 350-1 | >25.11 | >25.11 | >25 | ||
MMV1581341 | OSM-S-576 | EGT 351-1 | >25.11 | >25.11 | >25 | ||
MMV1581342 | OSM-S-577 | EGT 352-1 | >25.11 | >25.11 | >25 | ||
MMV1581344 | OSM-S-578 | EGT 353-1 | 1.36 | 1.288 | 1.324 | ||
MMV1581345 | OSM-S-579 | EGT 354-1 | >25.11 | >25.11 | >25 | ||
MMV1581346 | OSM-S-580 | EGT 355-1 | 1.397 | 1.026 | 1.212 | ||
MMV1581330 | OSM-S-581 | MK057-3 | >25.11 | >25.11 | >25 | ||
MMV1581331 | OSM-S-582 | MK079-1 | 0.393 | 0.357 | 0.375 | ||
MMV1581332 | OSM-S-583 | MK080-1 | >25.11 | >25.11 | >25 | ||
MMV1581333 | OSM-S-584 | MK081-1 | 1.059 | 0.968 | 1.014 | ||
MMV1581334 | OSM-S-585 | MK082-1 | 0.898 | 0.728 | 0.813 | ||
MMV1581343 | OSM-S-654 | EGT 338-1 | 0.121 | 0.112 | 0.117 |
@MFernflower for your question above, a quinone would not be consistent with the NMR and MS they obtained. We're pretty confident on it's structure.
The third batch for the year has just been sent out to Dundee for screening against P. falciparum. This is the largest set we've sent this year and it contains a number of key compounds.
UPDATE 6/7/18: Results are here.
Dundee Compounds 150618.zip