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Opentrons / opentrons

Software for writing protocols and running them on the Opentrons Flex and Opentrons OT-2
https://opentrons.com
Apache License 2.0
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bug: Offset of right pipette (P300 multichannel GEN2) #12165

Open RubenHitV opened 1 year ago

RubenHitV commented 1 year ago

Overview

After a factory reset and then successfully completing deck, pipette and tiplength calibration using software version 6.2.1 (but also some previous versions), the GEN2 P300 multichannel on our OT-2 has an offset when running newly created protocols using the OT-designer webapp (without Labware Position Check-mediated offsets) to the left and front of the exact same OT p300 tipbox in the same deckslot as used during calibration. The offset is of a magnitude that the tipbox sometimes tips over during runs, failing the rest of the protocol. Together with OT support, we have found a workaround whereby we use the LPC to correct this, but it would be nice if the calibration would be translated correctly to new protocols. However, we recently ran into problems with the labware position check as well. As the labware check is performed on the pipette installed on the left, and not on the right, this causes the major issue raised before.

Steps to reproduce

Perform calibration, run a protocol that uses the P300 multichannel in the same slot and watch if the orientation is correct.

Current behavior

The pipette is positioned to the left (~2-3mm) and front (~1mm) of where it should be, and where it was calibrated to be.

Expected behavior

Correctly translate the calibration data to a protocol run

Operating system

Windows

System and robot setup or anything else?

6.2.1 Wifi connection

RubenHitV commented 1 year ago

Allowing the option to do a Labware Position Check with the pipette installed on the right (not only the one on the left) would also solve this issue