When using the consolidate() function, if the volume is larger than 400uL, the robot doesn't break down the function into a series of transfer steps and just skips the step altogether.
Steps to reproduce
Code used to run:
from opentrons import protocol_api, types
metadata = {'apiLevel': '2.9'}
def run(protocol: protocol_api.ProtocolContext):
res = protocol.load_labware('nest_12_reservoir_15ml', '8',
'"12-Channel Reservoir (14mL DMEM in each of well 1, 2)"')
plate = protocol.load_labware('usascientific_96_wellplate_2.4ml_deep', '9',
'"96 Deep Well Plate w/ 16 working stocks in cols 1&2 + SFM bullet in A3"')
tip_rack_200 = protocol.load_labware('opentrons_96_filtertiprack_200ul', '11', '"Tip Rack 200 uL filtered"')
p300 = protocol.load_instrument('p300_single_gen2', 'right', [tip_rack_200])
p300.pick_up_tip()
# Consolidate breaks when the amount specified is greater than 400uL
p300.consolidate(
1000,
res.wells()[0],
plate.wells()[0],
new_tip='never')
p300.consolidate(
400,
res.wells()[0],
plate.wells()[0],
new_tip='never')
The same protocol was ran with 350 instead of 1000 in the consolidate function
Current behavior
When running the protocol through the app with 1000 in the consolidation step, the protocol will run normally and then skip the first consolidation step, but have no issues running 2nd consolidation step. With the volume as 350, the code runs all the way through, breaking down both consolidation functions as a series of smaller volume transfer steps.
Expected behavior
The software should break down the step with a volume of 1000 into a series of transfer functions of smaller volumes.
Overview
When using the consolidate() function, if the volume is larger than 400uL, the robot doesn't break down the function into a series of transfer steps and just skips the step altogether.
Steps to reproduce
Code used to run:
The same protocol was ran with 350 instead of 1000 in the consolidate function
Current behavior
When running the protocol through the app with 1000 in the consolidation step, the protocol will run normally and then skip the first consolidation step, but have no issues running 2nd consolidation step. With the volume as 350, the code runs all the way through, breaking down both consolidation functions as a series of smaller volume transfer steps.
Expected behavior
The software should break down the step with a volume of 1000 into a series of transfer functions of smaller volumes.