Thank you for this fantastic tool.
Just a minor suggestion: if the PAM is not detected in the list of sgRNAs, throw a warning.
I spent some time trying to figure out why the csv output did not contain the off-target PAM region when <Allow PAM edits> was TRUE. I now see that it was because my input sequences did not contain a PAM, and that this is written in the arguments specifications. It would have saved some stress if this was highlighted a little better, or if a warning was implemented :)
Thank you for this fantastic tool. Just a minor suggestion: if the PAM is not detected in the list of sgRNAs, throw a warning.
I spent some time trying to figure out why the csv output did not contain the off-target PAM region when
<Allow PAM edits>was TRUE. I now see that it was because my input sequences did not contain a PAM, and that this is written in the arguments specifications. It would have saved some stress if this was highlighted a little better, or if a warning was implemented :)