Hello! I have a follow-up query on the normalization question posed by @anncsun. Is converting data to logCPM, normalizing to TMM, and then standardizing required before running TALLSorts, or are these steps performed by the TALLSorts -s sample.csv function? Thank you!
Hey there, thanks for the question. Your sample.csv file should be a file of raw counts, and TALLSorts will do the normalisation/standardisation for you.
Hello! I have a follow-up query on the normalization question posed by @anncsun. Is converting data to logCPM, normalizing to TMM, and then standardizing required before running TALLSorts, or are these steps performed by the TALLSorts -s sample.csv function? Thank you!