The input mfe has to be divided by the length, this is ok for sequence under 40nt above that, there is no linearity applicable (On the Normalization of the Minimum Free Energy of RNAs by Sequence Length). There should a warning or sth like that.
Output: csv file with the normalized MFE:
"comment","normalizedmfe"
"Test123 457Test healp",-1.6611
"fefefe wefwe ffff",-1.725
R/Python Script Input: csv file with sequence length and the MFE:
The input mfe has to be divided by the length, this is ok for sequence under 40nt above that, there is no linearity applicable (On the Normalization of the Minimum Free Energy of RNAs by Sequence Length). There should a warning or sth like that.
Output: csv file with the normalized MFE: "comment","normalizedmfe" "Test123 457Test healp",-1.6611 "fefefe wefwe ffff",-1.725