BLAST against genome contigs not returning results

[ARWattam]

Even when I choose a single genome, nothing happens. I used the following sequence and chose BLAST against genome contigs, and I chose Brucella melitensis biovar abortus 2308. No results ever returned. When I do BLASTP with the same sequence (amino acids, though), that returns results.

fig|511145.12.peg.3032|b2938|VBIEscCol129921_3032| Biosynthetic arginine decarboxylase (EC 4.1.1.19) [Escherichia coli str. K-12 substr. MG1655 | 511145.12] atgagctcccaggaagccagcaagatgctgcgtacttacaatattgcctggtggggcaat aactactatgacgttaacgagctgggccacattagcgtgtgcccggacccggacgtcccg gaagctcgcgtcgatctcgcgcagttagtgaaaactcgtgaagcacagggccagcgtctg cctgcactgttctgtttcccacagatcctgcagcaccgtttgcgttccattaacgccgcg ttcaaacgtgcgagggaatcctacggctataacggcgattacttccttgtttatccgatc aaagttaaccagcaccgccgcgtgattgagtccctgattcattcgggcgaaccgctgggt ctggaagccggttccaaagccgagttgatggcagtactggcacatgctggcatgacccgt agcgtcatcgtctgcaacggttataaagaccgcgaatatatccgcctggcattaattggc gagaagatggggcacaaggtctatctggtcattgagaagatgtcagaaatcgccattgtg ctggatgaagcagaacgtctgaatgtcgttcctcgtctgggcgtgcgtgcacgtctggct tcgcagggttcgggtaaatggcagtcctccggcggggaaaaatcgaagttcggcctggct gcgactcaggtactgcaactggttgaaaccctgcgtgaagccgggcgtctcgacagcctg caactactgcacttccacctcggttcgcagatggcgaatattcgcgatatcgcgacaggc gttcgtgaatccgcgcgtttctatgtggaactgcacaagctgggcgtcaatattcagtgc ttcgacgtcggcggcggtctgggcgtggattatgaaggtactcgttcgcagtccgactgt tcggtgaactacggcctcaatgaatacgccaacaacattatctgggcgattggcgatgcg tgtgaagaaaacggtctgccgcatccgacggtaatcaccgaatcgggtcgtgcggtgact gcgcatcacaccgtgctggtgtctaatatcatcggcgtggaacgtaacgaatacacggtg ccgaccgcgcctgcagaagatgcgccgcgcgcgctgcaaagcatgtgggaaacctggcag gagatgcacgaaccgggaactcgccgttctctgcgtgaatggttacacgacagtcagatg gatctgcacgacattcatatcggctactcttccggcatctttagcctgcaagaacgtgca tgggctgagcagctttatttgagcatgtgccatgaagtgcaaaagcagctggatccgcaa aaccgtgctcatcgtccgattatcgacgagctgcaggaacgtatggcggacaaaatgtac gtcaacttctcgctgttccagtcgatgccggacgcatgggggatcgaccagttgttcccg gttctgccgctggaagggctggatcaagtgccggaacgtcgcgctgtgctgctggatatt acctgtgactctgacggtgctatcgaccactatattgatggtgacggtattgccacgaca atgccaatgccggagtacgatccagagaatccgccgatgctcggtttctttatggtcggc gcatatcaggagatcctcggcaacatgcacaacctgttcggtgataccgaagcggttgac gtgttcgtcttccctgacggtagcgtagaagtagaactgtctgacgaaggcgataccgtg gcggacatgctgcaatatgtacagctcgatccgaaaacgctgttaacccagttccgcgat caagtgaagaaaaccgatcttgatgctgaactgcaacaacagttccttgaagagttcgag gcaggtttgtacggttatacttatcttgaagatgagtaa

[ARWattam]

Here are the results when I do BLASTP with the amino acid version of the same sequence.

[mshukla1]

I tried

• BLASTN against reference genomes, it worked.
• BLASTN against E coli MG1655, it worked.
• tblastx against B 2308, it worked.

=> BLASTN against B 2308 didn't return any results, because there are no significant hits at DNA level.