PMID: 8641470 Site-directed mutagenesis of Saccharomyces cerevisiae beta-tubulin: Interaction between residue 167 and benzimidazole compounds.

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PHI-base curation

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PMID: 8641470 Site-directed mutagenesis of Saccharomyces cerevisiae beta-tubulin: Interaction between residue 167 and benzimidazole compounds. #131

Closed MPiovesana closed 1 year ago

MPiovesana commented 1 year ago

https://canto.phi-base.org/curs/1fcb305c3abf40f4

MPiovesana commented 1 year ago

Genotype annotation: authors replace endogenous copy of beta-tubulin with mutant copy containing point mutation F167Y (TUB2 (aaF167Y)).

MPiovesana commented 1 year ago

Phenotype annotation: I have suggested the addition of new terms to best describe the phenotypes observed for mutant lines (increased sensitivity to benomyl and normal sensitivity to thiabendazole). As this seems to be a requirement for several papers, perhaps we can discuss how to annotate these phenotypes without the need to include new terms (eg use term "increased sensitivity to chemical", do not pick a child term, and specify which chemical in Comments section).

AC We need the term 'normal growth on thiabendazole' We would use 'sensitive to benomyl' which is a child of 'increased sensitivity to chemical' and means that the mutant line is more sensitive to the chemical then the WT line.

remove NTR and leave as 'Sensitive to benomyl'

MPiovesana commented 1 year ago

Similarly, the chemical "nocodazole" is present in Experimental conditions, but not as a phenotype term.

MPiovesana commented 1 year ago

Cell growth phenotype annotation: authors report that mutation leads to an increased cell division time (reduced rate of cell division) of S. cerevisiae, assessed by counting cells. I looked up several phenotype terms and could not identify one to use here. Phenotype still to be annotated.

AC I have added below annotation