Closed MPiovesana closed 3 months ago
In this paper, the wild type strain GR5 of Aspergillus nidulans is transformed with an A. nidulans genomic library (autonomous non-integrating plasmid library created with genomic DNA from strain R153; strain origin is not mentioned in the paper, but in the reference where library creation is described - Osherov and May, 2000) with the aim to identify ORFs which confer resistance to the antifungal itraconazole. Itraconazole-resistant transformants were found to carry multiple copies of a plasmid harbouring the P-450 14-alpha demethylase gene pdmA, and plasmid eviction was associated with loss of antifungal resistance.
Uniprot ID: the Uniprot ID of pdmA of A. nidulans (Q9P462) was identified by searching UniprotKB with the GenBank accession number provided in the text (AF266481).
Genotypes and annotations: A transformant allele was created and named pdmA transformant (R153-pdmA), to indicate the expression of the pdmA gene derived from strain R153 in the transformed strain. "Ectopic" was selected under Expression level. Endogenous pdmA present was added in background (confirmed by Southern blot experiment in Fig 3).
AC- I think 'overexpression' is better than 'ectopic' in this case. I have changed genotypes. Text from abstract ![Uploading image.png…]()
PHIPO term "normal growth on amphotericin B" was suggested". Terms "resistance to itraconazole" and "resistance to fluconazole" were also used to annotate this genotype, all according to the results presented in Table (table is not numbered, but it is the only table in the paper so can be easily located).
Curation completed pending review.
I need to add new strain 'GR5' to Aspergillus nidulans in pathogen species list
Now done ec03253
Pathogen phenotype annotations look fine
Added GO MF
AE alteration in archetype is N/A as PdmA is not a FRAST listed target site (maybe could be similar to cyp51??) and no sequence difference was recorded in the genotype.
Session approved
Closing ticket
Curated by @MPiovesana https://canto.phi-base.org/curs/bf15e81bf1ab6473