PMID: 9758830 A novel ATP-binding cassette transporter involved in multidrug resistance in the phytopathogenic fungus Penicillium digitatum

[MPiovesana]

Curated by @MPiovesana https://canto.phi-base.org/curs/6b22874f745e874d#

[MPiovesana]

Straightforward paper where the role of the PMR1 gene of Penicillium digitatum in antifungal resistance was explored by gene disruption in a DMI-resistant strain. Strain LC2 (isolated from the surface of lemons) has a multidrug resistance phenotype, which was abolished with the disruption of PMR1.

[MPiovesana]

Uniprot ID: authors provide a GenBank accession number for PMR1 (AB010442) which was used to retrieve the Uniprot ID used in the curation session (O74637).

[MPiovesana]

Strain: strain LC2 was manually entered.

Genotype creation: a disruption allele named PMR1- (gene disruption) was created, and the expression level "Null" was selected as PMR1 transcript was undetectable (Northern blot, Figure 4) in the disruption line.

[MPiovesana]

Genotype annotation: the genotype was annotated with four PHIPO terms: sensitive to fenarimol sensitive to triflumizole (suggested) sensitive to bitertanol (suggested) sensitive to pyrifenox (suggested)

[MPiovesana]

Curation completed pending review.

[CuzickA]

Current annotations

[CuzickA]

Small edit to genotype

[CuzickA]

PHIPO and PHI-ECO terms already available now looks like this

[CuzickA]

Add new strain 5e762bc

[CuzickA]

AE alteration in archetype N/A as NTSR and disruption

Session approved

Closing ticket