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PHI-base curation
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PMID:11036010 Genetic analysis of azole resistance in the Darlington strain of Candida albicans #177

Closed MPiovesana closed 2 months ago

MPiovesana commented 1 year ago

Curated by @MPiovesana https://canto.phi-base.org/curs/8d279073acae6de3

MPiovesana commented 1 year ago

Mutations in the ERG11 gene of the azole-resistant Candida albicans strain Darlington were identified in this study, causing the amino acid substitutions Y132H and I471T. This mutant allele was cloned and introduced in the wild type recipient strain CAI4, and a transformant line heterozygous for the ERG11 gene replacement was identified. This line (transformant 12) carrying one wt and one mutant allele of ERG11 displayed increased resistance to fluconazole compared to the parental strain CAI4.

MPiovesana commented 1 year ago

Uniprot ID: a gene (ERG11) and species name (Candida albicans) search returned entry P10613.

AC 13_02_2024 UniProt looks good. Also used in https://github.com/PHI-base/curation/issues/172

Strain: strain CAI4 was selected as the recipient strain for transformation. (sensitive strain)

MPiovesana commented 1 year ago

Genotype creation: a transformant allele type was created named ERG11 transformant(Darlington-ERG11(Y132H I471T)) in the CAI4 background. A wild type allele ERG11+ was also created. Both alleles were combined in a heterozygous diploid genotype to represent the genotype of the CAI4 transformant isolated in this study.

MPiovesana commented 1 year ago

Genotype annotation: PHIPO term resistance to fluconazole was assigned to the heterozygous diploid genotype.

MPiovesana commented 1 year ago

Curation completed pending review.

CuzickA commented 9 months ago

Annotation looks fine.

AE alteration in archetype

ERG11+[WT level] / ERG11 transformant(Darlington-ERG11(Y132H I471T))[Not assayed] 'Y137H, I483T; ERG11 (Cyp51B); SEPTTR'

Could not find Candida albicans listed in Nichola's S files.

In Mair et al 2016 image image image

image

CuzickA commented 9 months ago

Next step

1) Check AE alteration in archetype with Nichola. 2) Also to double check that this is 'Cyp51B', authors call gene ERG11/Cyp51A1. ERG 11 uniprot is same as in https://github.com/PHI-base/curation/issues/172 where we decided that ERG 11 = Cyp51B

Then session ready to approve.

CuzickA commented 2 months ago

Session approved without Nichola checking AE alteration in archetype.

Closing ticket.