PMID:8702300 Isolation of a beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance

[MPiovesana]

Curated by @MPiovesana https://canto.phi-base.org/curs/446d4f792646ef99

[MPiovesana]

A benomyl-resistant mutant of Fusarium moniliforme was isolated from a UV-irradiation mutagenesis experiment with wild type strain A102. The fungicide target gene β-tubulin was cloned and sequences derived from the mutant and wild type strains were compared. A single mutation (Y50N) was identified. The benomyl resistance of the mutant strain was shown to be abolished at lower temperatures (15 degrees).

[MPiovesana]

Uniprot ID: the WT β-tubulin gene of F. moniliforme was deposited in GenBank with accession number U27303, which was used to retrieve ID P53374 in Uniprot.

Strain: wild type strain A102 (used for the UV mutagenesis experiment) was added to the session.

[MPiovesana]

Genotype creation: an amino acid substitution allele type was created to record the point mutation in the β-tubulin gene. Not assayed was selected as expression level (authors perform a Northern blot but they do not compare expression between WT and mutant strain).

[MPiovesana]

Genotype annotations: authors mention resistance to benomyl in the text, but the active compound added to growth media is MBC, which is carbendazim. Although benomyl is metabolised into carbendazim and, therefore, they could be considered synonymous, I thought the most accurate way to curate this experiment would be to add +carbendazim to experimental conditions, and use the PHIPO term 'resistance to carbendazim'. Thus, the following annotations were made:

resistance to carbendazim normal growth on carbendazim (suggested)

[MPiovesana]

Curation completed pending review.

[CuzickA]

Genotype annotations: authors mention resistance to benomyl in the text, but the active compound added to growth media is MBC, which is carbendazim. Although benomyl is metabolised into carbendazim and, therefore, they could be considered synonymous, I thought the most accurate way to curate this experiment would be to add +carbendazim to experimental conditions, and use the PHIPO term 'resistance to carbendazim'. Thus, the following annotations were made:

resistance to carbendazim normal growth on carbendazim (suggested)

This is tricky. I am going to change the annotations from carbendazim to benomyl as this is mentioned in the title and reported on by the authors. I will add a note to the comments that methyl-2-benzimidazole carbamate (MBC), the active ingredient of benomyl was added to the medium (and that methyl-2-benzimidazole carbamate = Carbendazim.)

[MPiovesana]

@CuzickA thank you for checking this. I was really in two minds about the best way to record this - if using the name of the compound which was actually added to the media or the more 'resistance to benomyl' term. I do agree that it makes more sense to go with the term used by the authors. Thanks for editing it!

[CuzickA]

This one is checked and just needs a new PHIPO term.

[CuzickA]

[CuzickA]

AE alteration_in _archetype

Y50N; TUB2 (β-tubulin); ASPEND

Straight forward to find in Nichola's S file in Table S1

[CuzickA]

updated genotype

[CuzickA]

AE alteration_in _archetype checked by Nichola. Session now approved.