PHI-base / curation

PHI-base curation
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Enable capturing of ‘Qualitative Gene Expression Data’ with interacting species adaptations in PHI-Canto. #74

Closed CuzickA closed 3 years ago

CuzickA commented 4 years ago

Wild-type Expression annotations could be made to the gene (not genotype/phenotype)

Discussed yesterday with @ValWood https://curation.pombase.org/pombase-trac/wiki/CapturingGeneExpression https://www.pombase.org/gene/SPBC11B10.09 look at section 'qualititive expression'

Eg in a WT pathogen-host interaction, recording that a pathogen and host gene are concurrently expressed eg EPI1 and P69B in PMID:15096512

Use this ticket to collect this data during curation. In the future the data can then be added back to the sessions once this function has been enabled.

Val suggested recording Syntax /controlled_curation="term=gene expression, RNA level; annotation_extension=during(GO:xxxxxxx); qualifier=increased; evidence=ECO:xxxx; db_xref=PMID:xxxxxxx; date=YYYYMMDD"

Will also need to adapt this for pathogen AND host interaction to document concurrent gene expression.

(There is also a bulk upload template PomBase use to capture gene expression data.)

CuzickA commented 4 years ago

For https://github.com/PHI-base/curation/issues/41 https://canto.phi-base.org/curs/babb18b437174938 The epi1 and p69B gene are concurrently expressed during infection of tomato by P. infestans. image Removed above from session and recorded in this ticket for the future.

CuzickA commented 4 years ago

https://github.com/pombase/canto/issues/1491

ValWood commented 4 years ago

I will chat with Kim about this next time I speak with him

jseager7 commented 3 years ago

The 'RNA level' annotation type should be enabled for genes on the main PHI-Canto server by tomorrow. It's already available on the demo server. Is that all you needed for this issue?

CuzickA commented 3 years ago

This works nicely thanks.

I have update the session https://canto.phi-base.org/curs/babb18b437174938

image

@jseager7 we will need to update the 'Help documentation'. I will also discuss with @martin2urban incorporating these new data types into the MC PHI5 display pages.

Closing ticket for now.

CuzickA commented 3 years ago

I was just looking at the AE options and when I select 'in presence of' see this error message

image

jseager7 commented 3 years ago

This is because we weren't loading the subset of the ChEBI ontology that PomBase uses for this annotation extension. I'm testing loading the ontology subset on the demo server now, and I'll close this issue once the ontology is available on the main server (probably tomorrow).

jseager7 commented 3 years ago

This is now fixed on the production server too. Closing.

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