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PNNL-Comp-Mass-Spec / Informed-Proteomics

Top down / bottom up, MS/MS analysis tool for DDA and DIA mass spectrometry data
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MSPathFinderT.exe crash #7

Closed somakray closed 6 years ago

somakray commented 6 years ago

Hello,

MSPathFinderT.exe crashes issuing the following error: what could have gone wrong?

Total Progress: 94.97%, 2d 14h 45.49m elapsed, Current Task:
Collected candidate matches: 0
Decoy database search elapsed Time: 109057.9 sec
Calculating spectral E-values for decoy-spectrum matches
Estimated matched sequences: 0
Decoy-spectrum match E-value calculation elapsed Time: 0.0 sec
Error computing FDR: Cannot compute FDR Scores; target file is empty
Error processing Frac4_140kR_01.raw: Cannot compute FDR Scores; target file is empty
FarmGeek4Life commented 6 years ago

It is saying that for some reason, it did not find any target database matches; is the _IcTarget.tsv file empty, or only have headers?

somakray commented 6 years ago

Yes, the '_IcTarget.tsv' contains only the headers. Maybe I should re-run with changed parameters in MSPathFinderT.exe to get some hits

FarmGeek4Life commented 6 years ago

That error message has now been changed to say Error computing FDR: Cannot compute FDR Scores; target results file is empty to make it more clear that it is referring to the file containing the target search results.