Open djinnome opened 1 year ago
This also has the benefit that during growth phase, Rhodo needs a nitrogen source, but during production phase, it is fine to produce bisabolene in a nitrogen starved state. Thus, by killing off azoto when a high enough Bisabolene titer has been reached, we automatically switch to nitrogen starved mode without any genetic modifications.
How can we model a "no growth/poison" signal in the models? Also, do we have the bisabolene reaction added to Rhodo?
EX_lyxL_e EX_homL_e EX_gluL_e EX_tre_e EX_arabL_e EX_leu__L_e EX_man_e EX_4abut_e EX_galt_e EX_dha_e
as carbon sources for RT all result in EX_glyclt_e, which AZ can use.
@djinnome Carbon sources for RT are above
@PBohutskyi Can you please check whether the binary culture grows in any of these carbon sources:
Carbon source to Rhodo | Rhodo byproduct to Azo |
---|---|
L-Homoserine | Glycolate |
L-Lyxose | Glycolate |
L-Glutamate | Glycolate |
L-Leucine | Glycolate |
Dihydroxyacetone | Glycolate |
D-Mannose | Glycolate |
Galactitol | Glycolate |
4-Aminobutanoate | Glycolate |
Trehalose | Glycolate |
L-Arabinose | Glycolate |
Rather than forcing Azo to eat what Rhodo is willing to part with, or vice versa, we can select two carbon sources, one of which only Rhodo can eat, and possibly one that only Azo can eat.
I say possibly, because when Rhodo grows, it produces Bisabolene, which may very well kill Azoto, so as long as Azoto can outcompete Rhodo for carbon source 2 early on, then the carbon will be available to Rhodo after Azoto dies.