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NTR Scr1 phosphorylated/ unphosphorylated #241

Closed ValWood closed 3 years ago

ValWood commented 3 years ago

scr1 S235A, S332A, S333A, S408A, S410A PMID:22140232 O14335

nataled commented 3 years ago

Hi Val, your terms are here!

PR:000050520 scr1/Phos:(S235/S332/S333/S408/S410) PR:000050521 scr1/UnPhos:(S235/S332/S333/S408/S410)

Question: is ECO:0005528 (site-directed mutagenesis phenotypic evidence)the right code for this? I'd like to start using something more specific than just "experimental". To that end, if you happen to know the correct code for each of the requests you make, please also include that information in future requests. Thanks!

[Term] id: PR:000050520 name: DNA-binding protein scr1 phosphorylated 1 (Schizosaccharomyces pombe) def: "A DNA-binding protein scr1 (Schizosaccharomyces pombe) that has been phosphorylated at positions equivalent to Ser-235, Ser-332, Ser-333, Ser-408, and Ser-410 of the amino acid sequence represented by UniProtKB:O14335. Example: UniProtKB:O14335, Ser-235/Ser-332/Ser-333/Ser-408/Ser-410, MOD:00046." [PRO:DAN, PomBase:VW, PMID:22140232] comment: Category=organism-modification. Requested by=PomBase. Kinase=("ssp2"; PR:000050522; Ser-235/Ser-332/Ser-333/Ser-408/Ser-410). Evidence=(ECO:0000006, based on PMID:22140232). synonym: "Spom-scr1/Phos:1" EXACT PRO-short-label [PRO:DAN] synonym: "UniProtKB:O14335, Ser-235/Ser-332/Ser-333/Ser-408/Ser-410, MOD:00046" NARROW PRO-proteoform-std [PRO:DAN] is_a: PR:000050519 ! DNA-binding protein scr1 (Schizosaccharomyces pombe) relationship: has_part MOD:00046 ! O-phospho-L-serine relationship: only_in_taxon NCBITaxon:4896 ! Schizosaccharomyces pombe

[Term] id: PR:000050521 name: DNA-binding protein scr1 unphosphorylated 1 (Schizosaccharomyces pombe) def: "A DNA-binding protein scr1 (Schizosaccharomyces pombe) that has not been phosphorylated at positions equivalent to Ser-235, Ser-332, Ser-333, Ser-408, and Ser-410 of the amino acid sequence represented by UniProtKB:O14335. Example: UniProtKB:O14335, Ser-235/Ser-332/Ser-333/Ser-408/Ser-410, PR:000026291." [PRO:DAN, PomBase:VW, PMID:22140232] comment: Category=organism-modification. Requested by=PomBase. synonym: "Spom-scr1/Phos:1" EXACT PRO-short-label [PRO:DAN] synonym: "UniProtKB:O14335, Ser-235/Ser-332/Ser-333/Ser-408/Ser-410, PR:000026291" NARROW PRO-proteoform-std [PRO:DAN] is_a: PR:000050519 ! DNA-binding protein scr1 (Schizosaccharomyces pombe) relationship: has_part MOD:00046 ! O-phospho-L-serine relationship: only_in_taxon NCBITaxon:4896 ! Schizosaccharomyces pombe

ValWood commented 3 years ago

site-directed mutagenesis phenotypic evidence sounds good!

will suggest evidence codes.