Term issue:

[ValWood]

I have previously requested phosphorylated/nonphsophorylated forms of proteins.

is it possible to get unphosphorylated hypophosphorylated (mitotic interphase) hyperphosphorylated (mitotic M-phase) forms of a protein/

Cdc11 (O74473) has 3 distinct forms throughout the cell cycle, but I don' yet know which residues. Also because there are so many phosphor sites we are unlikely to request residue-specific modified forms, but we could extend these items to indicate the collection of modified residues.

PMID:12546793

[nataled]

Hi Val, Based on a previous paper (PMID:11676915), what they call hypophosphorylated form (1) is actually fully unphosphorylated. I named the terms accordingly. Let me know if these meet your needs.

PR:000050568 = unphosphorylated (aka hypophosphorylated 1) PR:000050569 = hypophosphorylated (aka hypophosphorylated 2) PR:000050570 = hyperphosphorylated (aka hyperphosphorylated 3)

[Term]
id: PR:000050568
name: septation initiation network scaffold protein cdc11 unphosphorylated form (Schizosaccharomyces pombe)
def: "A septation initiation network scaffold protein cdc11 (Schizosaccharomyces pombe) that lacks phosphorylated residues." [PRO:DAN, PomaBase VW, PMID:12546793, PMID:11676915]
comment: Category=modification. Requested by=PomBase.
synonym: "Spom-cdc11/PhosRes-" EXACT PRO-short-label [PRO:DNx]
synonym: "cdc11p hypophosphorylated form (1) (Schizosaccharomyces pombe)" RELATED [PRO:DAN, PMID:12546793]
is_a: PR:000050566 ! septation initiation network scaffold protein cdc11 (Schizosaccharomyces pombe)
relationship: lacks_part MOD:00696 ! phosphorylated residue

[Term]
id: PR:000050569
name: septation initiation network scaffold protein cdc11 hypophosphorylated form (Schizosaccharomyces pombe)
def: "A septation initiation network scaffold protein cdc11 (Schizosaccharomyces pombe) that includes at least one phosphorylated residue, and is the predominant phosphorylated form observed during interphase." [PRO:DAN, PomBase:VW, PMID:12546793]
comment: Category=modification. Requested by=PomBase.
synonym: "Spom-cdc11/HypoPhos+" EXACT PRO-short-label [PRO:DNx]
synonym: "cdc11p hypophosphorylated form (2) (Schizosaccharomyces pombe)" RELATED [PRO:DAN, PMID:12546793]
synonym: "cdc11p interphase form (Schizosaccharomyces pombe)" RELATED [PRO:DAN, PMID:12546793]
is_a: PR:000050566 ! septation initiation network scaffold protein cdc11 (Schizosaccharomyces pombe)
relationship: has_part MOD:00696 ! phosphorylated residue

[Term]
id: PR:000050570
name: septation initiation network scaffold protein cdc11 hyperphosphorylated form (Schizosaccharomyces pombe)
def: "A septation initiation network scaffold protein cdc11 (Schizosaccharomyces pombe) that includes phosphorylated residues at a level greater than the interphase-observed phosphorylated form, and is only observed during mitotic anaphase." [PRO:DAN, PomBase:VW, PMID:12546793]
comment: Category=modification. Requested by=PomBase. Note: Dependent upon the activity (unknown if direct or indirect) of cdc7p [PMID:12546793] and other factors. The interphase-observed form is PR:000050569.
synonym: "Spom-cdc11/HyperPhos+" EXACT PRO-short-label [PRO:DNx]
synonym: "cdc11p hyperphosphorylated form (3) (Schizosaccharomyces pombe)" RELATED [PRO:DAN, PMID:12546793]
is_a: PR:000050566 ! septation initiation network scaffold protein cdc11 (Schizosaccharomyces pombe)
relationship: has_part MOD:00696 ! phosphorylated residue            

[ValWood]

This sounds good, val