PacificBiosciences / FALCON

FALCON: experimental PacBio diploid assembler -- Out-of-date -- Please use a binary release: https://github.com/PacificBiosciences/FALCON_unzip/wiki/Binaries
https://github.com/PacificBiosciences/FALCON_unzip/wiki/Binaries
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Can't start assembly jobs for the latest version of falcon #671

Closed fayina closed 6 years ago

fayina commented 6 years ago

Hello I installed the latest version of falcon on my cluster and setting the parameters in cfg file suit for my genome(fish, 1.5~1.7G), but the program can't start succesfull and with no erro information in all.log. I don't know where is wrong in my cfg file or any thing else. This is my config file:

[General] job_type=lsf input_fofn = input.fofn input_type = raw length_cutoff = 10000 length_cutoff_pr = 15000 stop_all_jobs_on_failure = False target = assembly jobqueue = Sharedqueue sge_option_da = -n 5 -q %(jobqueue)s sge_option_la = -n 6 -q %(jobqueue)s sge_option_cns = -n 6 -q %(jobqueue)s sge_option_pda = -n 6 -q %(jobqueue)s sge_option_pla = -n 7 -q %(jobqueue)s sge_option_fc = -n 6 -q %(jobqueue)s

pa_concurrent_jobs = 200 cns_concurrent_jobs = 200 ovlp_concurrent_jobs = 200 pa_HPCdaligner_option = -v -B188 -t16 -w8 -M24 -e.75 -k18 -l4000 -s1000 ovlp_HPCdaligner_option = -v -B188 -t24 -M24 -k24 -h180 -e.96 -l2800 -s1000

pa_DBsplit_option = -x1000 -s250 -a ovlp_DBsplit_option = -x1000 -s250 falcon_sense_option = --output_multi --min_idt 0.70 --min_cov 4 --max_n_read 300 --n_core 8 overlap_filtering_setting = --max_diff 60 --max_cov 70 --min_cov 1 --bestn 10 --n_core 12 use_tmpdir = true use_tmpdir = /YZGROUP4/STORAGE/genewiz/genewiznj/Public

and this is all.log: all.log

this is mypwatcher dir output image

pb-cdunn commented 6 years ago

I installed the latest version of falcon

Meaning, a binary release? Something from Bioconda? This repo is out-of-date, as we no longer mirror our internal code-base here.

You should always test integration with the tiniest example possible. There is "synth0" in FALCON-examples for this purpose.