I tried to assemble a Candida interspecies hybrid (diploid) under the assumption that alleles differ by ~5% using FALCON-Unzip. I tried different assemblers and so far the FALCON-Unzip assembly appears to be the most promising. What is bugging me is that only one of two telomeres at the end of each contig gets assembled. In this organism, telomeres appear to be 1-2 kbp long repetitive sequences with a very high content of G and T (>70%). I tried various different parameter settings but I am just not able to get both telomeres. Here is the config file for the best assembly so far:
In total ~6 Gbp were sequenced and I assume that the diploid genome size is ~32Mbp. Thus, the average coverage is ~187.5X. What am I doing wrong? How should i set parameters in order to get both telomeres assembled?
I tried to assemble a Candida interspecies hybrid (diploid) under the assumption that alleles differ by ~5% using FALCON-Unzip. I tried different assemblers and so far the FALCON-Unzip assembly appears to be the most promising. What is bugging me is that only one of two telomeres at the end of each contig gets assembled. In this organism, telomeres appear to be 1-2 kbp long repetitive sequences with a very high content of G and T (>70%). I tried various different parameter settings but I am just not able to get both telomeres. Here is the config file for the best assembly so far:
In total ~6 Gbp were sequenced and I assume that the diploid genome size is ~32Mbp. Thus, the average coverage is ~187.5X. What am I doing wrong? How should i set parameters in order to get both telomeres assembled?