PacificBiosciences / FALCON_unzip

Making diploid assembly becomes common practice for genomic study
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Falcon Unzip crashed at 0-phasing (blasr ?) #81

Open agroppi opened 7 years ago

agroppi commented 7 years ago

I'm using the last version of falcon-unzip after an assembly with the lastest version of falcon. I have blasr 5.3 python 2.7.12 The first step 3-unzip/reads ended but at the 0-phasing step Directories are created (here is an exemple : 0-phasing/000011F) But after that the pipeline stops Here is my cfg file :

[General]
job_type = local

[Unzip]
# list of fasta files
input_fofn = /mnt/cbib/sWAGMAN/Marouch/Falcon_0.5_Marouch/input_Marouch_falcon_0.5.fofn
# input_bam_fofn= input_bams.fofn

# path to bin directory containing samtools, blasr, and various GenomicConsensus utilities
smrt_bin=/module/apps/samtools/1.4/bin/ /module/apps/blasr/5.3/blasr/blasr /module/apps/python/2.7.12/bin/ /home/ag/GenomicConsensus/bin/

sge_phasing= --nodes 1 --ntasks-per-node 6
sge_quiver= --nodes 1 --ntasks-per-node 10
sge_track_reads= --nodes 1 --ntasks-per-node 6
sge_blasr_aln= --nodes 1 --ntasks-per-node 10
sge_hasm= --nodes 1 --ntasks-per-node 20
unzip_concurrent_jobs = 20
quiver_concurrent_jobs = 20

and here attached the all.log : all.log.txt

nottwy commented 7 years ago

Can you check if there is a file called '000191F_reads.fa' under the directory 3-unzip/reads/ ?

agroppi commented 7 years ago

Hi YES there is a file called 000191F_reads.fa under the directory 3-unzip/reads/

nottwy commented 7 years ago

You can attach your file '3-unzip/0-phasing/000191F/blasr/pwatcher/stderr' here.

agroppi commented 7 years ago

I don't have 3-unzip/0-phasing/000191F/blasr/pwatcher/stderr In the directory 3-unzip there is only the directory reads

But in the directory readsI have a symbolic link to mypwatcher/jobs/P4e06e95c523470/stderr

Here is this file : stderr.zip

nottwy commented 7 years ago

You have to change the name of your fofn file into input.fofn. Unzip only supports this name so fat. It didn't parse the name in your unzip.cfg.

agroppi commented 7 years ago

Thanks for your quick answer. I've changed the names of my config and fofn files I've just relaunched Falcon Unzip I'll keep you informed