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PacificBiosciences / kineticsTools

Tools for detecting DNA modifications from single molecule, real-time sequencing data
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Update README.md #71

Closed GDelevoye closed 3 years ago

GDelevoye commented 4 years ago

From my knowledge, old Sequel I Data can perfectly be analyzed with kineticsTools. All the models, including the latest S2-P2 are present in the "resources" directory.

natechols commented 4 years ago

Technically this is accurate for the version currently here, but support for older chemistries has been removed in the PB repo (not yet mirrored here).

rhallPB commented 4 years ago

Note also, S2-P2 model has been shown to be effective with Sequel II chemistry version 2.0. We don't have a good model for Sequel II chemistry version 1.0.

GDelevoye commented 4 years ago

Thank you both for answering.

I must say that the README is a bit confusing. I am a PhD student and I did an analysis 2 years ago on Sequel I data with kineticsTools. By comming back on Github I was afraid that I didn't use the right software

@rhallPB, so just to be sure... The model you would recommand to use with both Sequel I Data (C4-P6 chemistry) and Sequel II (v2 chemistry) Data is the model SP2-C2, am I right ? This is what I understood from your answer and from 694c265b990351918b48e4e15d12944f33f422ea