PacificBiosciences / pbbioconda

PacBio Secondary Analysis Tools on Bioconda. Contains list of PacBio packages available via conda.
BSD 3-Clause Clear License
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Question: does pbmm2 modify MM and ML tags for supplementary alignments with hard clippings? #621

Closed lh3 closed 9 months ago

lh3 commented 9 months ago

@armintoepfer, here is a pbmm2 question for you. PacBio now outputs MM/ML tags for methylation. When pbmm2 outputs a supplementary alignment with hard clippings, does it modify the original MM/ML, copy them over to the output SAM line, or discard MM/ML on hard clipped SAM line? For the context, see samtools/hts-specs#646, but you don't have to read the thread.

armintoepfer commented 9 months ago

Hey @lh3, pbmm2 never hard clips. All of our alignments are either primary or supplementary with soft clippings.

lh3 commented 9 months ago

Got it. Thanks!