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PacificBiosciences / pbbioconda

PacBio Secondary Analysis Tools on Bioconda. Contains list of PacBio packages available via conda.
BSD 3-Clause Clear License
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FL_TPM #718

Open ngs-seqer opened 2 months ago

ngs-seqer commented 2 months ago

Hi PacBio,

I just checked a filtered isoform list in "XXX.filtered_lite_classification.txt" file from my isoseq3/pigeon pipeline. There are many, so I want to prioritize the predicted isoform transcripts.

I found the "FL_TPM" column. Can this column be considered "isoform quantification"?

If not, what could be the best way to sort/prioritize transcripts? FALAIR or IsoQuant (I do not have short-read RNA-seq data).

Let me know what you think!

armintoepfer commented 2 weeks ago

@Magdoll can you please chime in?