error in Trinity

[dbajpp0]

Here is Joan Pons. I run full pipeline in conda environment in linux manjaro ./nextflow run TransPi.nf --all --reads "/dades/jpp/rna_plantes/*_R[1,2].uniq.fastq.gz" --k 25,41,53 --maxReadLen 150 -resume

config file was set to skipNormalization = true Command output: ERROR, don't recognize parameter: --no_normalize_reads Please review usage info for accepted parameters. I checked Trintity and subcommand --no_normalize_reads do not exist. I guess this command was set by the pipeline istself Any tip? Thanks

[dbajpp0]

I solved the issue by using a newer version of Trinity

[rivera10]

Hello @dbajpp0, Did you install the tools manually? Or did you use the precheck script?

[dbajpp0]

Hi Ramon,

I installed manually.

Joan

On 04/09/2021 19:29, Ramon Rivera wrote:

Hello @dbajpp0 https://github.com/dbajpp0, Did you install the tools manually? Or did you use the precheck script?

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[rivera10]

Ok, thanks for the info. I was worried the pipeline was not creating the env correctly. Anything let me know.