PalMuc / TransPi

TransPi – a comprehensive TRanscriptome ANalysiS PIpeline for de novo transcriptome assembly
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Fail to download uniprot_metazoa_33208.fasta #56

Closed dppss90008 closed 1 year ago

dppss90008 commented 1 year ago

Dear author

I found the following error, when i ran the test command ./nextflow run TransPi.nf --all -profile docker,test --outdir Results_test

image

the raising error showed that uniprot_metazoa_33208.fasta is missing.

I traced back to the database, and found the following error. The error may due to the api changes in uniprot database, when I ran precheck_TransPi.sh image

hope you can help with this issue.

sevragorgia commented 1 year ago

Dear dppss90008,

please check issue #52

the rest api changed and you have to use a legacy option. The instructions to solve this issue can be found there (in issue #52)

cheers

Sergio

dppss90008 commented 1 year ago

Dear Sergio,

I tried replacing the old URL with "https://legacy.uniprot.org/uniprot/?query=taxonomy:33208&format=fasta&compress=yes&include=no".

But it still not work. It seems that the URL you provided is no longer valid.

CH

sevragorgia commented 1 year ago

Hmm, I was afraid this will be case at some point but not now :-(

I will try to figure out how to do it now but I may need some time. Will post it back here.

Sergio

sevragorgia commented 1 year ago

I drafted a possible solution in issue #52. Please report if it worked.