results different between FastANI and ANI result from Kostas lab

ParBLiSS / FastANI

Fast Whole-Genome Similarity (ANI) Estimation

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results different between FastANI and ANI result from Kostas lab #13

Closed sze1217 closed 6 years ago

sze1217 commented 6 years ago

Hi,

i tried using FastANI to do multiple genomes comparison and one of the genome show no any similarity to another genome which is quite weird as they are closely related organism, so I tried using another ANI calculator from kostas lab to do and it show 95% similarity, may I know the reason behind as it changes a lot the output??

Thanks!

cjain7 commented 6 years ago

Haven't noticed such a case before. Would it be possible for you to share those two genomes with me for debugging purpose?

sze1217 commented 6 years ago

I guess it's fine now, the genomes I used are without ORF prediction, guess it would be better to run thru prediction then only compare the genomes ANI. Thanks!