ParBLiSS / FastANI

Fast Whole-Genome Similarity (ANI) Estimation
Apache License 2.0
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Some missing results in output file. #78

Open spencer411 opened 3 years ago

spencer411 commented 3 years ago

I am analyzing a dataset that consists of uncharacterized streptococcus isolates downloaded from NCBI. Fastani executes fine with no error message, yet my output file is missing a large number of comparisons. See my heatmap attached. Initially, I was running this via SLURM, but looking at past similar issues I have tried running it directly from the command line, I have also tried re-installing it, yet I keep getting the same results. Note that it was installed using conda and I have double-checked that both miniconda 3 and all base packages are up to date as well. Note that I have also screened this dataset for contamination with checkm, and I am not analyzing any genomes with more than 5% contamination. I have analyzed a couple of other datasets with no issues. Any assertation as to what might be going on is greatly appreciated.

Screen Shot 2020-10-08 at 1 54 56 PM
cjain7 commented 3 years ago

Please see #64 (may be a those pairs correspond to distant genomes) You could run AAI and confirm