bead normalising when less beads in some tubes

[stefk7]

Hi, I had an issue with the beads in the first batch of my mass cytometry experiment. The beads were sticking to the plastic tube and therefore not enough were in solution of my sample to normalise my data properly and this is why I am using premessa to bead normalise my data. The bead normalised graphs premessa is producing look different in batch 1 to the ones being produced for my other 2 batches (which had enough beads). The slope of the graphs Is still straightening out after normalising but they look different, does anyone know if they are still being normalised properly?

[SamGG]

Hi, No answer for you. You may look at a well expressed marker such as CD3 or CD45 versus time and check if the signal is stable or still decreasing. The best would be to clearly label the batches on the screenshot and post your question on cytoforum where you will get help from the whole community. Issues in github are more dedicated to software questions (installations, bugs...). Best, Samuel