Open eric-hy opened 1 year ago
Hi @eric-hy , sorry for the delay. To call methylation using ccsmeth, the input data (hifi.bam) must contain the kinetics signals (i.e., containing TAGs such as fi
, fp
,ri
, rp
in each item of the bam file). Also, please add --no_decode
in the call_mods command for the Revio dataset. For more information, pleash check issue #40 .
Best, Peng
Dear authors, thanks for your great tool 'ccsmeth'. I try calling modifications on a hifi.bam generated from Pacbio Revio platform. However, I only get a empty bam files. I have noticed the given models are trained using PacBio Sequel II reads, I wonder whether differences between the two platforms leads to my results. I look forward to your reply!