Hi Jason,
This workflow looks great and I hope to try it out soon.
If you haven't already considered it, you might want to take a look at incorporating this tool as an alternative/replacement for LiftOff.
I particularly like the feature it has to annotate the identity/variation type relative to the source annotation.
https://github.com/Kuanhao-Chao/LiftOn
Kua-Hao Chao, Jakob M. Heinz, Celine Hoh, Alan Mao, Alaina Shumate, Mihaela Pertea, and Steven L. Salzberg. "Combining DNA and protein alignments to improve genome annotation with LiftOn." bioRxiv, doi: https://doi.org/10.1101/2024.05.16.593026, 2024.
Description of feature
Hi Jason, This workflow looks great and I hope to try it out soon. If you haven't already considered it, you might want to take a look at incorporating this tool as an alternative/replacement for LiftOff. I particularly like the feature it has to annotate the identity/variation type relative to the source annotation. https://github.com/Kuanhao-Chao/LiftOn Kua-Hao Chao, Jakob M. Heinz, Celine Hoh, Alan Mao, Alaina Shumate, Mihaela Pertea, and Steven L. Salzberg. "Combining DNA and protein alignments to improve genome annotation with LiftOn." bioRxiv, doi: https://doi.org/10.1101/2024.05.16.593026, 2024.