Create: HCN linamarase

[ontology-variable-submission-app[bot]]

Submitter

Name	Lizbeth Pino Duran
Program	CIAT
Email	l.pino@cgiar.org

Creation Request

Trait Name	HCN linamarase
Proposed Synonyms	HCN linamarase (ug/g wb)
Definition	The cyanogenic potential of extracts from cassava roots (or other cassava-based products), evaluated by reaction with linamarase followed by colorimetric determination of cyanide concentration and expressed in ug/g of sample wet basis
Trait Class	Quality
Measurement Method	Cassava roots (or other samples: skin, leaves, flour, etc.) are ground into a mash and cyanogenic compounds extracted using a buffer of orthophosphoric acid. The extracts are incubated with linamarase to release CN ions, which are quantified by spectrophotometry at 605 nm (in presence of chloramin T and isonicotinic/1,3 dimethyl-barbituric reagent).
Measurement Type	Unit
Measurement Unit	Number

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