Open JuanQuirosCR opened 2 months ago
the method to measure ETR is based on the pulse amplitude modulation (PAM) technique. It basically consists on emitting blue light pulse (over a leaf area of about 1cm2) which will cause chlorophyll fluorescence (ChlF); such ChlF information is used to estimate the quantum efficiency of photosystem II (Y II) and subsequently ETR as a proxy of photosynthesis.
The ETR data is collected every 15min during the day and every 2 hours during the evening, and we normally acquire such data in 50-100 plants in a time window of 2-4 weeks.
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