search

PreibischLab / BigStitcher

ImgLib2/BDV implementation of Stitching for large datasets
GNU General Public License v2.0
68 stars 15 forks source link

Off by one error in DoG detection? #122

Open lacan opened 1 year ago

lacan commented 1 year ago

Tried to perform a DoG on a large amount of DAPI tiles that have some 3D to them. Some Z are empty and not others, which was leading to a strange behaviour that makes me think that the Z slice index is off by one.

to reproduce and see Import a bunch of tiles, make sure that they do not start or end at the same Z slice, this helps to view Alternatively, I guess a couple stacks with very different points-like objects in Z would work. Then you would think that the LoG detector is not working, as the detected rois won't overlap with the point objects on the image.

Here is a small GIF

offbyonebigstitcher

I am using the latest version as per the Fiji Update Site of BigStitcher.

lacan commented 1 year ago

Hello, Should I provide further information?

StephanPreibisch commented 1 year ago

Hi, could you please send me the image? I didn't find an obvious bug ...