Open Xqua opened 6 years ago
Hi @Xqua,
I assume the problem is related to the tiles we introduced with BigStitcher. Since we automatically move Views in x,y,z according to their metadata (to have a good pre-alignment with tiled images), your Angles are probably moved in a way that they don't overlap (and the registration will ignore non-overlapping images). I can reproduce the same problem in a test dataset I have lying around (BigDataViewer screenshot):
We are actively working on fixing this issue (e.g. 996411f3dafd5b9f76401fe724f0ef0369f78bfd), so I hope the problem will be gone with the next update in Fiji.
In the meantime, you should be able to fix this by selecting Registration in between views: Compare all views against each other
(instead of Only overlapping views
as shown below) Basic Registration Parameters dialog.
I hope that you should then be able to register the images.
If that does not help, as a last resort, you can uninstall BigStitcher to downgrade to the previous version of MVR ( 😢 ).
Best, David
Thanks for the info ! I understand what's happening now :D
Couldn't make it work even by asking it map each to each ...
went back to the pre MVR version and it's working like a charm !
Happy to provide you with a CZI for debugging if you ever need one !
Hi @Xqua,
Another solution (if you do not want to downgrade ;) ) would be to remove the metadata translation from the view registrations. Depending on whether you used the Z.1 Loader or the AutoLoader, you could do the following:
Z.1 Loader:
apply rotation to dataset
when opening the .cziRemove Transformation -> Newest/Latest transformation
. Alternatively, if you are using batch mode, you can use Plugins -> MVR -> Batch Processing -> Tools -> Apply Transformations
in the Fiji menu and apply an identity model on top of the calibration.AutoLoader:
Then, the MVR should work like in the old version (but with the various tweaks and optimizations we introduced since then).
I'll try to reproduce the error you got on the dataset I have lying around (I should be pretty much the same as yours, a simple, multi-angle czi with one image per angle). If it's easy for you to provide your file, I can have a look as well, thanks for the offer!
Best, David
Hi since the new version that detects tiles I'm running into this issue when I want to register the dataset:
The problem is that I have no tiles in my dataset to start with.