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PreibischLab / multiview-reconstruction

Software for the reconstruction of multi-view microscopic acquisitions like Selective Plane Illumination Microscopy (SPIM) Data
GNU General Public License v2.0
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Detections found interactively but then 0 detections found #13

Open lacan opened 6 years ago

lacan commented 6 years ago

We have a sample with very weak beads that we want to detect. We wanted to subsequently thin out the detections to remove the sample.

But as the title says. We find the detections when working on the DoG detector interactively, but afterwards, when we let the program run, there are no detections... Even on the image we used to test the detector.. Any thoughts where I could look into to fix this?

StephanPreibisch commented 6 years ago

Hi, a few screenshots would be helpful ...

lacan commented 6 years ago

example sorry for the delay

Best Oli

StephanPreibisch commented 6 years ago

Hi, maybe you could send me two example views so I can have a look?

Alyesca commented 6 years ago

Hallo Stephan

Thanks so much for getting back to me! I have checked the website bettest points (look fine to me) and the calibration. The angle arrangement looks ok, too, and I’ve tried different registration models.

I have uploaded the nucleus channel view 1 and 2 here: https://cloudstor.aarnet.edu.au/plus/s/Yvb7bWhxMPMRvld

The voxel size etc is in the photo below.

Very curious to hear what you think!

Danke sehr und beste Gruesse aus down under, Verena

[image1.jpeg]

On 14 Sep 2018, at 6:24 pm, Stephan Preibisch notifications@github.com<mailto:notifications@github.commailto:notifications@github.com> wrote:

Hi, maybe you could send me two example views so I can have a look?

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On 14 Sep 2018, at 6:24 pm, Stephan Preibisch notifications@github.com<mailto:notifications@github.com> wrote:

Hi, maybe you could send me two example views so I can have a look?

— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHubhttps://github.com/PreibischLab/multiview-reconstruction/issues/13#issuecomment-421270975, or mute the threadhttps://github.com/notifications/unsubscribe-auth/ApIE-CEezBi5v8C-xZJJ2efq7-YwJzCCks5ua2eigaJpZM4Vv3bP.

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