olso commented 6 years ago

Existing Equipment {docsify-ignore-all}

[x] Microscope Levenhuk D740T trinocular Link
- [x] Microscope slides (~76x26x1mm) (100pcs) Link (code 5362)
- [x] Slides top (~22x22mm) (200pcs) Link

Nice laboratory equip shops

co2 supply
Spectrometer
Petri dishes
- Between 80 and 140mm
Glasswear
- 500ml beakers
- Closable jars
Chemicals
- 70% ethanol solution, disinfectant
Vaha
Personal protective equipment
- Coats
- Glasses
- Gloves
Various microscopes *
Visualiser
- for glowing at visible wavelength
Electrophoresis tank and power supply
Centrifuge
Agarose
Pipettes
- 10, 20, 50, 100ul volume
- Adjustable pipette (1ul increments)
- Pipette tips
HEPA filter workbench
- Laminar hood
- https://wiki.london.hackspace.org.uk/view/Project:Flow_Hood
Sterile manipulation box
Light source (for growing)
Thermal cycler (OpenPCR)
- http://openpcr.org
- https://en.wikipedia.org/wiki/Thermal_cycler
- http://www.instructables.com/id/Arduino-PCR-thermal-cycler-for-under-85/
Autoclave
- Test
- Autoclave tape
- Microwave
- Pressure cooker?
- For sterilizing labware and bio material
Incubator
- Old egg incubator?
- Pet heating pads with thermostat
Refigerator freezer
- 4C
- -20C
- -80C freezer
Consumables
Molecular biology enzymes and reagents
Non-pathogenic ecoli bactieria
Nitrile gloves
liquid tubes for measuring
Bacterial transformation buffer

vviiz4rd commented 6 years ago

Chceme čo sa dá DIY ? E.g. centrifúgy a tak ?

olso commented 6 years ago

@vviiz4rd Yup, check this thing out http://sphere.diybio.org/

There is literally everything needed to set this up, actually