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Przemol / seqplots

:chart_with_upwards_trend:SeqPlots - An interactive tool for visualizing NGS signals and sequence motif densities along genomic features using average plots and heatmaps.
http://przemol.github.io/seqplots
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Normalization #21

Closed jawmajewski closed 6 years ago

jawmajewski commented 8 years ago

Hi,

Great tool and a lot of fun to play with. Could you add an option to normalize by total tags? Right now it is very difficult to compare profiles from different size datasets (and the Z-score normalization does not really help)

J

Przemol commented 6 years ago

The BW tracks need to be normalised by external software.

For BAM it's theoretically possible to include such option, but I would still recommend using specialised software, like rBEADS: https://github.com/Przemol/rbeads

It does a proper normalisation, in addition to sequencing depth balancing.