RGLab / CytoML

A GatingML Interface for Cross Platform Cytometry Data Sharing
GNU Affero General Public License v3.0
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Problems with CytoML package #114

Closed Chrissi92 closed 4 years ago

Chrissi92 commented 4 years ago

Hello together, I would like to evaluate my Flowjo data in R, and so I came across the CytoML package.

However, I have some troubles with it. I tried to install the packages and load the libraries, but I am always getting error messages, even when I try different options.

It would be so great if you could check the code and if you would have any suggestions.

Thank you very much in advance.

Best, Christina

Option1:

if (!requireNamespace("BiocManager", quietly = TRUE))

  • install.packages("BiocManager")

BiocManager::install("CytoML") Bioconductor version 3.11 (BiocManager 1.30.10), R 4.0.2 (2020-06-22) Installing package(s) 'CytoML' package �CytoML� is in use and will not be installedInstallation path not writeable, unable to update packages: MASS, mgcv, nlme, survival Old packages: 'glue' Update all/some/none? [a/s/n]: a cannot open URL 'https://bioconductor.org/packages/3.11/data/annotation/bin/windows/contrib/4.0/PACKAGES.rds': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/data/annotation/bin/windows/contrib/4.0/PACKAGES.gz': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/data/experiment/bin/windows/contrib/4.0/PACKAGES.rds': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/data/experiment/bin/windows/contrib/4.0/PACKAGES.gz': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/workflows/bin/windows/contrib/4.0/PACKAGES.rds': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/workflows/bin/windows/contrib/4.0/PACKAGES.gz': HTTP status was '404 Not Found'versuche URL 'https://cran.biodisk.org/bin/windows/contrib/4.0/glue_1.4.2.zip' Content type 'application/zip' length 155551 bytes (151 KB) downloaded 151 KB

package ‘glue’ successfully unpacked and MD5 sums checked cannot remove prior installation of package �glue�Problem C:\R-4.0.2\R_LIBS_USER\00LOCK\glue\libs\x64\glue.dll nach C:\R-4.0.2\R_LIBS_USER\glue\libs\x64\glue.dll zu kopieren: Permission deniedrestored �glue� The downloaded binary packages are in C:\Users\aulehner\AppData\Local\Temp\Rtmpmg0ZoC\downloaded_packages

if (!requireNamespace("BiocManager", quietly = TRUE))

  • install.packages("BiocManager")

BiocManager::install("flowWorkspace") Bioconductor version 3.11 (BiocManager 1.30.10), R 4.0.2 (2020-06-22) Installing package(s) 'flowWorkspace' cannot open URL 'https://bioconductor.org/packages/3.11/data/annotation/bin/windows/contrib/4.0/PACKAGES.rds': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/data/annotation/bin/windows/contrib/4.0/PACKAGES.gz': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/data/experiment/bin/windows/contrib/4.0/PACKAGES.rds': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/data/experiment/bin/windows/contrib/4.0/PACKAGES.gz': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/workflows/bin/windows/contrib/4.0/PACKAGES.rds': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/workflows/bin/windows/contrib/4.0/PACKAGES.gz': HTTP status was '404 Not Found'versuche URL 'https://bioconductor.org/packages/3.11/bioc/bin/windows/contrib/4.0/flowWorkspace_4.0.6.zip' Content type 'application/zip' length 8455899 bytes (8.1 MB) downloaded 8.1 MB

package ‘flowWorkspace’ successfully unpacked and MD5 sums checked cannot remove prior installation of package �flowWorkspace�Problem C:\R-4.0.2\R_LIBS_USER\00LOCK\flowWorkspace\libs\x64\flowWorkspace.dll nach C:\R-4.0.2\R_LIBS_USER\flowWorkspace\libs\x64\flowWorkspace.dll zu kopieren: Permission deniedrestored �flowWorkspace� The downloaded binary packages are in C:\Users\aulehner\AppData\Local\Temp\Rtmpmg0ZoC\downloaded_packages Installation path not writeable, unable to update packages: MASS, mgcv, nlme, survival Old packages: 'glue' Update all/some/none? [a/s/n]: a cannot open URL 'https://bioconductor.org/packages/3.11/data/annotation/bin/windows/contrib/4.0/PACKAGES.rds': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/data/annotation/bin/windows/contrib/4.0/PACKAGES.gz': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/data/experiment/bin/windows/contrib/4.0/PACKAGES.rds': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/data/experiment/bin/windows/contrib/4.0/PACKAGES.gz': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/workflows/bin/windows/contrib/4.0/PACKAGES.rds': HTTP status was '404 Not Found'cannot open URL 'https://bioconductor.org/packages/3.11/workflows/bin/windows/contrib/4.0/PACKAGES.gz': HTTP status was '404 Not Found'versuche URL 'https://cran.biodisk.org/bin/windows/contrib/4.0/glue_1.4.2.zip' Content type 'application/zip' length 155551 bytes (151 KB) downloaded 151 KB

package ‘glue’ successfully unpacked and MD5 sums checked cannot remove prior installation of package �glue�Problem C:\R-4.0.2\R_LIBS_USER\00LOCK\glue\libs\x64\glue.dll nach C:\R-4.0.2\R_LIBS_USER\glue\libs\x64\glue.dll zu kopieren: Permission deniedrestored �glue� The downloaded binary packages are in C:\Users\aulehner\AppData\Local\Temp\Rtmpmg0ZoC\downloaded_packages

library(CytoML) library(flowWorkspace) As part of improvements to flowWorkspace, some behavior of GatingSet objects has changed. For details, please read the section titled "The cytoframe and cytoset classes" in the package vignette:

vignette("flowWorkspace-Introduction", "flowWorkspace")

manual_gating_xml = system.file("extdata",

  • package = "flowWorkspaceData",
  • "manual.xml") ws = openWorkspace(manual_gating_xml) Fehler: openWorkspace is now deprecated by CytoML::open_flowjo_xml. Please library(CytoML) first!

Option 2:

install_conditional <-function(pkg, source = c("Bioc", "CRAN", "RGLab")) {

  • source <- match.arg (source, c("Bioc", "CRAN", "RGLab"))
  • utils::chooseCRANmirror(ind = 64)
  • for (p in kg) {
  • if ( source == "CRAN") {
  • if (is.na(match(p, installed.packages()[, "Package"]))) {
  • install.packages(p, ask = FALSE)
  • } else {
  • update.packages(p)
  • }
  • } else if (source == "Bioc") {
  • BiocManager::install(p, ask = FALSE, update = FALSE, type = "source")
  • } else {
  • devtools::install_github(paste("RGLab", p, sep = "/"), ref = "trunk")
  • }
  • }
  • } install_conditional(
  • c(
  • "MLmetrics",
  • "webshot",
  • "Gmisc",
  • "rsvg",
  • "DiagrammeR",
  • "DiagrammeRsvg",
  • "BiocManager",
  • "devtools",
  • "cowplot",
  • "png",
  • "grid",
  • "gridExtra",
  • "gtable"
  • ),
  • source = "CRAN"
  • ) Fehler in install_conditional(c("MLmetrics", "webshot", "Gmisc", "rsvg", : Objekt 'kg' nicht gefunden devtools::install_github("RGLab/cytolib@v2.0.3") Fehler: .onLoad in loadNamespace() für 'pkgload' fehlgeschlagen, Details: Aufruf: NULL Fehler: Paket ‘backports’ hat keinen Namensraum devtools::install_github("RGLab/RProtoBufLib@v1.3.7") Fehler: .onLoad in loadNamespace() für 'pkgload' fehlgeschlagen, Details: Aufruf: NULL Fehler: Paket ‘backports’ hat keinen Namensraum devtools::install_github("RGLab/flowCore@v1.3.2") Fehler: .onLoad in loadNamespace() für 'pkgload' fehlgeschlagen, Details: Aufruf: NULL Fehler: Paket ‘backports’ hat keinen Namensraum devtools::install_github("RGLab/flowWorkspace@v3.29.7") Fehler: .onLoad in loadNamespace() für 'pkgload' fehlgeschlagen, Details: Aufruf: NULL Fehler: Paket ‘backports’ hat keinen Namensraum devtools::install_github("RGLab/openCyto@v1.19.2") Fehler: .onLoad in loadNamespace() für 'pkgload' fehlgeschlagen, Details: Aufruf: NULL Fehler: Paket ‘backports’ hat keinen Namensraum devtools::install_github("RGLab/CytoML@v1.7.10") Fehler: .onLoad in loadNamespace() für 'pkgload' fehlgeschlagen, Details: Aufruf: NULL Fehler: Paket ‘backports’ hat keinen Namensraum devtools::install_github("RGLab/CytoML@v1.7.10") Fehler: .onLoad in loadNamespace() für 'pkgload' fehlgeschlagen, Details: Aufruf: NULL Fehler: Paket ‘backports’ hat keinen Namensraum devtools::install_github("RGLab/ggcyto@v1.9.12") Fehler: .onLoad in loadNamespace() für 'pkgload' fehlgeschlagen, Details: Aufruf: NULL Fehler: Paket ‘backports’ hat keinen Namensraum library(CytoML) Registered S3 method overwritten by 'data.table': method from print.data.table
    Fehler: package or namespace load failed for ‘CytoML’ in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]): Namensraum ‘cytolib’ 1.3.2 ist geladen, aber >= 2.0.3 wird benötigt devtools::install_github("RGLab/CytoML@v2.0.3") Fehler: .onLoad in loadNamespace() für 'pkgload' fehlgeschlagen, Details: Aufruf: NULL Fehler: Paket ‘backports’ hat keinen Namensraum
gfinak commented 4 years ago

Your first error:

Installing package(s) 'CytoML'
package �CytoML� is in use and will not be installed Installation path not writeable, unable to update packages: MASS, mgcv, nlme, survival

suggests (amongst other things) that you don't have write permission to update your system.

Your next error:

cannot open URL 'https://bioconductor.org/packages/3.11/data/annotation/bin/windows/contrib/4.0/PACKAGES.rds': HTTP status was '404 Not Found'cannot open URL

suggests your system is somehow misconfigured. The URL to the PACKAGES.rds file should look like: https://bioconductor.org/packages/3.11/bioc/bin/windows/contrib/4.0/PACKAGES.rds

This is not a CytoML or cytoverse problem. This is a problem with your system.