Closed EplisonG closed 3 years ago
The warning tells you that by importing "All Samples" you are making a GatingSet that contains samples with different gating schemes attached. Generally importing "All Samples" is not recommended because of this.Import your different sample groups separately into separate Gating Sets.
The warning tells you that by importing "All Samples" you are making a GatingSet that contains samples with different gating schemes attached. Generally importing "All Samples" is not recommended because of this.Import your different sample groups separately into separate Gating Sets.
Thank you very much for your kind reply, Greg. I change the name to other samples ( for example 2 or 3):
gt.samp2 <- flowjo_to_gatingset(ws, name = 2)
gt.samp3<- flowjo_to_gatingset(ws, name = 3)
The warning message still appears. could it be a problem with the manually gated flowjo data? Thanks.
It simply means that the samples in each of your sample groups are not consistently gated. They have different gating schemes in some way. It's advised to organize your sample groups so that samples in a group have a common gating scheme. I'm pretty sure this is even the use case for sample groups in FlowJo.
It simply means that the samples in each of your sample groups are not consistently gated. They have different gating schemes in some way. It's advised to organize your sample groups so that samples in a group have a common gating scheme. I'm pretty sure this is even the use case for sample groups in FlowJo.
It's quite clear to me. I will go to check the gating strategies in FlowJo. Thank you, Greg. I truly appreciate it.
Hi,
I'm just starting to learn flowjo and how to use CytoML. After opening the workspace with:
I would like to read gates from FlowJo workspace with:
However, A warning message appears:
I try to find what the problem was but did not have clues. Could anyone give me some suggestions or hints? Thank you very much.
-Nancy