In the last two years I have been using warpSet to normalize my flow cytometry data and I have used this line of code
fs <- warpSet(fs, stains = antigen[c(3,4,5,9,17,18,20)])
where
antigen[c(3,4,5,9,17,18,20)]
are the channels I want to normalize
[1] "CD8" "GZMK" "GZMB" "Ki67" "Tbet" "TCF1" "Eomes"
Lately I am getting the following error:
Estimating landmarks for channel Eomes ... Registering curves for parameter CD8 ... Error in landmarkreg(fdobj, landmarks, WfdPar = WfdPar, monwrd = monwrd, : unused argument (monwrd = monwrd)
Could you please help me understanding why?
Thanks!
Hello!Thanks for flowStats!
In the last two years I have been using warpSet to normalize my flow cytometry data and I have used this line of code
fs <- warpSet(fs, stains = antigen[c(3,4,5,9,17,18,20)])
where
antigen[c(3,4,5,9,17,18,20)]
are the channels I want to normalize[1] "CD8" "GZMK" "GZMB" "Ki67" "Tbet" "TCF1" "Eomes"
Lately I am getting the following error:
Estimating landmarks for channel Eomes ... Registering curves for parameter CD8 ... Error in landmarkreg(fdobj, landmarks, WfdPar = WfdPar, monwrd = monwrd, : unused argument (monwrd = monwrd)
Could you please help me understanding why? Thanks!