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RGLab / flowWorkspace

flowWorkspace
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problem boolean filter #320

Closed luigidolcetti closed 4 years ago

luigidolcetti commented 4 years ago

Hi,

so, on a flowframe I have created some gates to clean up the FACS data and run FLOWSOM pulling out a number of subpopulations, I used the flow som matrix to feed flowworkspace gating set with new populations (everything works fine until this point). Now I am using again boolean filter to merge FLOWSOM population in clusters, but getting the statistics returns only NA. Same happens doing filters on the pre-FLOWSOM gates (even repeating the same OR gate that I used to feed FLOWSOM). Statistics on all the pre-exsisting gates work anyway. The gating set has been saved/loaded via save_gs. Any suggestion where to look for some clues?

Best regard, Luigi

mikejiang commented 4 years ago

Make sure you run recompute(gs) after you add gates/filters to GatingSet. If it still doesn't work, please paste your example code for troubleshooting

luigidolcetti commented 4 years ago

shame on me! Thank you!

From: Mike Jiang notifications@github.com Sent: 13 April 2020 18:43 To: RGLab/flowWorkspace flowWorkspace@noreply.github.com Cc: Dolcetti, Luigi luigi.dolcetti@kcl.ac.uk; Author author@noreply.github.com Subject: Re: [RGLab/flowWorkspace] problem boolean filter (#320)

Make sure you run recompute(gs) after you add gates/filters to GatingSet. If it still doesn't work, please paste your example code for troubleshooting

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