Gating using a specific quadrant

RGLab / openCyto

A package that provides data analysis pipeline for flow cytometry.

GNU Affero General Public License v3.0

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Gating using a specific quadrant #223

Closed drpreeti3628 closed 4 years ago

drpreeti3628 commented 4 years ago

Hi,

I have created a quadrant gate for CD4 and CD8 populations using the following logic "7","*","+/-+/-","CD3+","CD4,CD8","gate_mindensity","gate_range = c(8,8)",NA,NA,"",NA

This created 4 quadrants. Now I wanted to create a further child quadrant gate say from Q1 of the above quadrant gate. What should be parent . Because the above parent was *.

Can you please help

mikejiang commented 4 years ago

See the doc here http://bioconductor.org/packages/release/bioc/vignettes/openCyto/inst/doc/HowToWriteCSVTemplate.html#13_pop_=_%E2%80%9C+-+-%E2%80%9D which explains how * is expanded into 4 separate population rows

mikejiang commented 4 years ago

i.e. you will have CD3+/CD4-CD8+, CD3+/CD4+CD8+,CD3+/CD4+CD8-,CD3+/CD4-CD8- generated and to be referred as parents for subsequent gating

drpreeti3628 commented 4 years ago

Thanks it worked.