How to add correct gate_range

[drpreeti3628]

Hi,

I created a quadrant gate and then picked on the quadrant as parent to further gate using gate_mindensity as gatign method and setting a range using gate_range arguments.

I want to leverage the mindensity at x axis at 8 and and at y axis at 2.5. But whatever option I try it leverages only one value at both X and Y axis. Can you please suggest a better option

"6","CD45RACD62L","+/-+/-","PE-A-CAR-BUV396-A-CD5+","PerCP-Cy5-5-A-CD45RA,BV605-A-CD62L","gate_mindensity","gate_range = c(8,2.5)",NA,NA,"",NA

[mikejiang]

You will have to do one axis at a time/row, e.g.

*	+/-	PE-A-CAR-BUV396-A-CD5+	cd45	gate_mindensity	gate_range = 8
*	+/-	PE-A-CAR-BUV396-A-CD5+	cd62	gate_mindensity	gate_range = 2.5

[gfinak]

Thanks, Mike. @drpreeti3628, The gate_range argument (and all gating arguments) passed straight through to each gating method. We aren't doing anything clever with the gating arguments in the expansion.

[mikejiang]

Actually this will be closer to your goal if quadGate on cd45 & cd62 are what you wanted

cd45_gate	+	PE-A-CAR-BUV396-A-CD5+	cd45	gate_mindensity	gate_range = 8
cd45_gate	+	PE-A-CAR-BUV396-A-CD5+	cd62	gate_mindensity	gate_range = 2.5
*	+/-+/-	PE-A-CAR-BUV396-A-CD5+	cd45,cd62	refGate	cd45_gate:cd62_gate

[drpreeti3628]

Thanks @mikejiang and @gfinak it worked.

I leveraged 2 single gates followed by refGate and it gave me accurate results.

Thank you so much