APAlyzer is a toolkit for bioinformatic analysis of alternative polyadenylation (APA) events using RNA sequencing data. Our main approach is the comparison of sequencing reads in regions demarcated by high-quality polyadenylation sites (PASs) annotated in the PolyA_DB database (https://exon.apps.wistar.org/PolyA_DB/v3/). The current version (v3.0) uses RNA-seq data to examine APA events in 3’ untranslated regions (3’UTRs) and in introns. The coding regions are used for gene expression calculation.
The bam files downloaded from the download_testbam() seemed corrupted.
"[E::bgzf_read] Read block operation failed with error 33 after 0 of 4 bytes
[E::bam_hdr_read] Invalid BAM binary header
[main_samview] fail to read the header from "Testis_rep1.bam".
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The bam files downloaded from the download_testbam() seemed corrupted.
"[E::bgzf_read] Read block operation failed with error 33 after 0 of 4 bytes [E::bam_hdr_read] Invalid BAM binary header [main_samview] fail to read the header from "Testis_rep1.bam". "