Open Rohit-Satyam opened 2 years ago
Hi, Sorry you had to face the issue. I can see that you have mentioned the gene identifier as ID. Can you please check if any of the entries is missing an ID identifier? PERF uses a in house script for parsing GFF and GTF files and maybe facing an issue. Thank you for the suggestion on integrating AGAT with PERF. I'll surely look into it.
Hi, Based on you input files I have downloaded the genome and GFF of "Tenacibaculum_discolor" from NCBI and run PERF on it.
PERF -i GCF_003664185.1_ASM366418v1_genomic.fna.gz -g GCF_003664185.1_ASM366418v1_genomic.gff.gz --gene-key ID
Using length cutoff of 12
Processing NZ_RCCS01000003.1: 100%|██████████████████████| 12/12 [00:00<00:00, 18.09it/s]
Generating annotations for identified repeats..
100%|██████████████████████████████████| 2759/2759 [00:00<00:00, 32419.89it/s]
NZ_RCCS01000004.1 1021 1033 AAAATT 12 - 2 TTTAAT gene-C8N27_RS00345427 1491 - Genic Promoter -594
NZ_RCCS01000004.1 1452 1466 AACAC 14 - 2 TGTGT gene-C8N27_RS00345427 1491 - Genic Promoter -1025
NZ_RCCS01000004.1 2143 2155 AAAATG 12 - 2 CATTTT gene-C8N27_RS003501668 4418 - Genic Promoter -475
NZ_RCCS01000004.1 2301 2313 AAACG 12 - 2 TTCGT gene-C8N27_RS003501668 4418 - Genic Promoter -633
Seems to have not faced any issue. Can you please check your input file.
Hi !!
I was trying to use the updated version of PERF and use the new feature for one of my bacterial strains. However, I am getting the following error
ERROR:
My GFF files contains the following flags in last column but changing it to
ID
or any other flag isn't workingWhen I use GTF file the error is
I am not sure what is being used in the background to process GFF/GTF files but my highest recommendation is to integrate PERF with AGAT which is an excellent tool for GTF/GFF file processing and handling.