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RNA-FRETools / MASH-FRET

MATLAB package to analyze single-molecule FRET data
https://rna-fretools.github.io/MASH-FRET/
GNU General Public License v3.0
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MASH-FRET bug report #67

Closed Befazl closed 3 years ago

Befazl commented 4 years ago

Describe the bug A clear and concise description of what the bug is.

While video processing, it is not possible to put in the coordinates via coordination file (...ace.coord) I tried with newly generated coordination files, as well as with coordination files generated with older MASH versions. Both resulted in the same error message.

To Reproduce Steps to reproduce the behavior:

  1. Go to Video processing
  2. Click "Load..." to upload a movie file (.sif)
  3. Click on "Input coordinates" in the intensity integration panel
  4. See error

Error Code 09:21 -- Load coordinates ... Index in position 1 exceeds array bounds (must not exceed 1).

Error in orgCoordCol (line 44) if x_col(i,1)>nCol || y_col(i,1)>nCol

Error in pushbutton_TTgen_loadCoord_Callback (line 43) coord_itg = orgCoordCol(fDat, 'cw', p.itg_impMolPrm, p.nChan, ...

Error while evaluating UIControl Callback.

Expected behavior Upload of the coordination file

Screenshots If applicable, add screenshots to help explain your problem.

Desktop (please complete the following information):

Additional context I have also applied the routine tests integrated in MASH to check for all functionalities, where further Errors were generated. Please see attached word file. 20200924_MASH_bug report.docx

Your contact besim.fazliji@chem.uzh.ch

mca-sh commented 4 years ago

Hello Befazl, I see that you are using the MASH-FRET-17 branch, which is in construction and not usable for now. Please use the MASH-FRET-16 branch, or download the last version here: https://github.com/RNA-FRETools/MASH-FRET/archive/MASH-code-16.zip. Please tell me if you still get issues with this version.

Befazl commented 4 years ago

Dear mca-sh,

Thank you a lot for your answer. I have tested also with the MASH-FRET-16 branch, and I received the exact same error messages. I have also downloaded and tested with MASH prev.commit e6bd182 which was uploaded 4 days ago, unfortunately with the same result, except that the routine testing for the Transition analysis was now successful. For all others, I received the exact same error. Unfortunately, I also cannot upload the coordination file in video processing, since also here I get the exact same error as before.

Thanks you a lot for your help.

mca-sh commented 4 years ago

Hello Befazl,

Mmm.. I am confused since in my case, importing .coord files causes no error and the interface testing gives no error and asks nothing from the user. I am sorry if this is a too trivial remark, but did you remove the MASH-code-17 from the search path of MATLAB (or delete it from your computer) before running MASH-code-16? Also, may I have your .coord file? Thanx!

EDIT: Also, is the number of channels in panel Experiment settings correctly set before you import the .coord file?

mca-sh commented 3 years ago

The issue was solved so I close it.