Closed rootze closed 1 year ago
Hi @rootze , thanks for reporting this. It looks like echolocatoR
(via the subpackage echoconda
) is having trouble finding the conda executable. You can see the error message at the end of each locus report:
Specified conda binary '/data/homezvol0/zechuas/.cache/R/basilisk/1.8.1/0/bin/conda' does not exist.Locus MEX3C complete in: 1.06 min
conda should automatically be installed for you by echoconda
if it hasn't been already, so I'm not sure why that's not happening. Could you please share your session info?
I was trying to find some tutorials to follow for this newest version of echolocatoR, so I found this r-markdown. https://github.com/RajLabMSSM/echolocatoR/blob/master/vignettes/echolocatoR.Rmd
This is indeed the latest tutorial. The documentation website is rather behind as it's tied to the GitHub Actions workflow which is not completing atm, but I will try to get this updated soon.
@bschilder Thanks for helping out. Here is the sessionInfo(). It seems that my conda env echolocator has [29] echoconda_0.99.7
.
> sessionInfo()
R version 4.2.1 (2022-06-23)
Platform: x86_64-conda-linux-gnu (64-bit)
Running under: Rocky Linux 8.6 (Green Obsidian)
Matrix products: default
BLAS/LAPACK: /data/homezvol0/zechuas/.conda/envs/echolocator/lib/libopenblasp-r0.3.21.so
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] echolocatoR_2.0.1
loaded via a namespace (and not attached):
[1] utf8_1.2.2 reticulate_1.26
[3] R.utils_2.12.0 tidyselect_1.2.0
[5] RSQLite_2.2.18 AnnotationDbi_1.58.0
[7] htmlwidgets_1.5.4 grid_4.2.1
[9] BiocParallel_1.30.4 XGR_1.1.8
[11] munsell_0.5.0 codetools_0.2-18
[13] interp_1.1-3 DT_0.25
[15] withr_2.5.0 colorspace_2.0-3
[17] OrganismDbi_1.38.1 Biobase_2.56.0
[19] filelock_1.0.2 knitr_1.40
[21] supraHex_1.34.0 rstudioapi_0.14
[23] stats4_4.2.1 DescTools_0.99.46
[25] MatrixGenerics_1.8.1 GenomeInfoDbData_1.2.8
[27] mixsqp_0.3-43 bit64_4.0.5
[29] echoconda_0.99.7 basilisk_1.8.1
[31] vctrs_0.5.0 generics_0.1.3
[33] xfun_0.33 biovizBase_1.44.0
[35] BiocFileCache_2.4.0 R6_2.5.1
[37] GenomeInfoDb_1.32.4 AnnotationFilter_1.20.0
[39] bitops_1.0-7 cachem_1.0.6
[41] reshape_0.8.9 DelayedArray_0.22.0
[43] assertthat_0.2.1 BiocIO_1.6.0
[45] scales_1.2.1 nnet_7.3-18
[47] rootSolve_1.8.2.3 gtable_0.3.1
[49] lmom_2.9 ggbio_1.44.1
[51] ensembldb_2.20.2 rlang_1.0.6
[53] MungeSumstats_1.4.5 echodata_0.99.14
[55] splines_4.2.1 lazyeval_0.2.2
[57] rtracklayer_1.56.1 gargle_1.2.1
[59] dichromat_2.0-0.1 hexbin_1.28.2
[61] checkmate_2.1.0 BiocManager_1.30.18
[63] yaml_2.3.6 reshape2_1.4.4
[65] snpStats_1.46.0 backports_1.4.1
[67] GenomicFeatures_1.48.4 ggnetwork_0.5.10
[69] Hmisc_4.7-1 RBGL_1.72.0
[71] tools_4.2.1 echoplot_0.99.5
[73] ggplot2_3.3.6 ellipsis_0.3.2
[75] catalogueR_1.0.0 RColorBrewer_1.1-3
[77] proxy_0.4-27 BiocGenerics_0.42.0
[79] coloc_5.1.0.1 Rcpp_1.0.9
[81] plyr_1.8.7 base64enc_0.1-3
[83] progress_1.2.2 zlibbioc_1.42.0
[85] purrr_0.3.5 RCurl_1.98-1.9
[87] basilisk.utils_1.8.0 prettyunits_1.1.1
[89] rpart_4.1.16 deldir_1.0-6
[91] viridis_0.6.2 S4Vectors_0.34.0
[93] SummarizedExperiment_1.26.1 ggrepel_0.9.1
[95] cluster_2.1.4 fs_1.5.2
[97] crul_1.3 magrittr_2.0.3
[99] data.table_1.14.4 echotabix_0.99.8
[101] dnet_1.1.7 openxlsx_4.2.5
[103] mvtnorm_1.1-3 ProtGenerics_1.28.0
[105] matrixStats_0.62.0 hms_1.1.2
[107] patchwork_1.1.2 XML_3.99-0.11
[109] jpeg_0.1-9 readxl_1.4.1
[111] IRanges_2.30.1 gridExtra_2.3
[113] compiler_4.2.1 biomaRt_2.52.0
[115] tibble_3.1.8 crayon_1.5.2
[117] R.oo_1.25.0 htmltools_0.5.3
[119] echoannot_0.99.7 tzdb_0.3.0
[121] Formula_1.2-4 tidyr_1.2.1
[123] expm_0.999-6 Exact_3.2
[125] DBI_1.1.3 dbplyr_2.2.1
[127] MASS_7.3-58.1 rappdirs_0.3.3
[129] boot_1.3-28 Matrix_1.5-1
[131] readr_2.1.3 piggyback_0.1.4
[133] cli_3.4.1 R.methodsS3_1.8.2
[135] echofinemap_0.99.3 parallel_4.2.1
[137] igraph_1.3.5 GenomicRanges_1.48.0
[139] pkgconfig_2.0.3 GenomicAlignments_1.32.1
[141] dir.expiry_1.4.0 RCircos_1.2.2
[143] foreign_0.8-83 osfr_0.2.9
[145] xml2_1.3.3 XVector_0.36.0
[147] echoLD_0.99.7 stringr_1.4.1
[149] VariantAnnotation_1.42.1 digest_0.6.30
[151] graph_1.74.0 httpcode_0.3.0
[153] Biostrings_2.64.1 cellranger_1.1.0
[155] htmlTable_2.4.1 gld_2.6.5
[157] restfulr_0.0.15 curl_4.3.3
[159] Rsamtools_2.12.0 rjson_0.2.21
[161] lifecycle_1.0.3 nlme_3.1-160
[163] jsonlite_1.8.2 viridisLite_0.4.1
[165] BSgenome_1.64.0 fansi_1.0.3
[167] downloadR_0.99.4 pillar_1.8.1
[169] susieR_0.12.27 lattice_0.20-45
[171] GGally_2.1.2 googleAuthR_2.0.0
[173] KEGGREST_1.36.3 fastmap_1.1.0
[175] httr_1.4.4 survival_3.4-0
[177] glue_1.6.2 zip_2.2.1
[179] png_0.1-7 bit_4.0.4
[181] Rgraphviz_2.40.0 class_7.3-20
[183] stringi_1.7.8 blob_1.2.3
[185] latticeExtra_0.6-30 memoise_2.0.1
[187] dplyr_1.0.10 irlba_2.3.5.1
[189] e1071_1.7-11 ape_5.6-2
Ok, so I'm noticing the basilisk
version is a bit older (1.8.1 vs. 1.9.12):
https://github.com/LTLA/basilisk/blob/master/DESCRIPTION
I've encountered a couple of issues with the earlier versions of basilisk
, but not sure if it's related to your issue as well.
I also just noticed that your Bioc version is a bit outdated (latest release is 3.16):
The selected method (rsamtools) depends on Rhtslib, and the version you have installed (1.28.0) contains known bugs. Please install Rhtslib >=1.99.2 via Bioconductor >=3.16:
BiocManager::install(version='devel')
2: In system(paste(act.cmd, collapse = " "), intern = TRUE) :
So I think the best thing to do would be upgrade to Bioc 3.16, which should automatically upgrade your basilisk
version as well.
BiocManager::install(version = "3.16")
In fact, I think upgrading to Bioc 3.16 will circumvent your need for conda at all, since echotabix
adaptively chooses the best available method given your R package versions. The "ramstools" method (which is R native) tends to be much more robust than "conda" method (which is partly a command line wrapper for conda). Not that you need to worry about those details, this is all handled automatically under the hood.
Just noting that this might also relate to this convo: https://github.com/LTLA/basilisk/issues/16
@bschilder Updating BiocManager
and basilisk
solved the empty result problem of echolocatoR::finemap_loci. Thank you so much!
@bschilder Just a minor request for the tutorial when you have time to update it. I also saw your closed issue posted under PAINTOR. It would be great to know how we can perform the fine mapping with annotation in echolocatoR. I am currently reading your function reference page and source code to figure it out. But there are quite some parameters that we can explore. For example, I would like to fine-mapping with annotation .bed files, as shown in https://github.com/gkichaev/PAINTOR_V3.0/wiki/2b.-Overlapping-annotations So it would be great to have an example from you.
@rootze no problem, glad it worked!
Regarding PAINTOR
, I'm actually in the midst of trying to use those exact annotations provided by the PAINTOR
team. The problem, it's a massive set of files that would ideally by queryable for relevant subsets. Once I get that down, I'll work on getting custom user-supplied annotations as an option as well (a feature I'm also working on for PolyFun atm).
You can follow the progress on this front here:
https://github.com/RajLabMSSM/echofinemap/issues/14
Also, just a side note, the empty results can occur any time there is an error inside the multi-locus iterator bc I have a tryCatch
built-in. This allows users to keep fine-mapping lots of loci even when a couple of them fail for whatever unforeseen reason. But to turn this off and get a better look at the trackback messages, set:
finemap_loci(use_tryCatch = FALSE, ...)
Hello @bschilder Thank you for instructing me on the installation of the echolocatoR in my last GitHub issue. Great work again. Love the image after loading the echolocatoR library. π¦ π¦ π¦ e c h o l o c a t o R π¦ π¦ π¦
I was trying to find some tutorials to follow for this newest version of echolocatoR, so I found this r-markdown. https://github.com/RajLabMSSM/echolocatoR/blob/master/vignettes/echolocatoR.Rmd
However, I have some problems with getting results.
I am not so sure if there is anything wrong with the above message when running
echolocatoR::finemap_loci
But here are the things in my R env, and the returned result following the code you provided in that link is a list of BST1, MEX3C, and merged_dat, but all are empty. Is that what we expected, or is something wrong here?Thank you so much for your time and help here.
rootze