Rappsilber-Laboratory / build-xiview

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Error message #91

Closed Suparat-Scheu closed 8 months ago

Suparat-Scheu commented 2 years ago

Hi! I'm trying to analyse my data but got the error message following ;

log id: gnvv5kgj7hrkipqfn1ih0nmsj3_1661331176.log (include this in the github issue) error type: Error message: invalid literal for float(): 1,005 id: undefined

Could anyone help me point out where to fix ? Thank you

colin-combe commented 2 years ago

i think its probably because you have commas in the number fields of a csv file you uploaded? It doesn't like that. Try with decimal points marked with a "." ?

Suparat-Scheu commented 2 years ago

So far I noticed the commas in the number fields in the first analysis and I didn't modify my first file after I got the results. The analysis did work with E.coli See this data file:

20220819_Ecoli_xiSEARCH_2nd_run_Xi1.7.6.7_CSM_xiFDR2.1.5.5.csv

So I did the same with P. vulgatus but it showed up error I mentioned. Exp02_Pvulgatus_1mM_BS3_Xi1.7.6.7_CSM_xiFDR2.1.5.5.csv

Could you point out which column I should fix? Thank you

colin-combe commented 2 years ago

which column I should fix?

the comma was being used as a thousands separator (in all columns i think) but it was in the PepPos columns that it particularly didn't like it. i fixed it in all of them: Exp02_Pvulgatus_1mM_BS3_Xi1.7.6.7_CSM_xiFDR2.1.5.5_2.csv

could this have happened because of saving it from Excel?

Suparat-Scheu commented 2 years ago

Thank you Colin for a fixed file. Hmm.. I don't think so because I did not modify the file before uploading in the Xi-view.

colin-combe commented 2 years ago

I don't think so because I did not modify the file before uploading in the Xi-view

Ok. So, it came out of xiFDR this way? (Just double checking this.) ( @lutzfischer )

Suparat-Scheu commented 2 years ago

Hi Coli, I took the data directly from the folder called "FDR_rpfdr5.0" after I proceed with Xi-Search. I go this data because I clicked on " do FDR" on the parameter tab so I didn't perform the analysis in XiFDR. As a new user, would you recommend to process the data from Xi-search to Xi-FDR again even I already selected "do FDR" in the parameter tab of Xi-search? Thank you

colin-combe commented 2 years ago

hi - yes, it seems other people have had this problem also.

it should be possible to remove the commas with the 'number format' settings in your spreadsheet tool

the FDR you did should be fine i think (though xiFDR might remove those commas), but xiFDR does provide some more options about how the FDR is done